Silver-doped bioactive glass fibres as a potential treatment for wound-associated bacterial biofilms.
Autor: | Shirgill S; University of Birmingham, School of Dentistry, 5 Mill Pool Way, Birmingham, West Midlands, B5 7EG, United Kingdom., Poologasundarampillai G; University of Birmingham, School of Dentistry, 5 Mill Pool Way, Birmingham, West Midlands, B5 7EG, United Kingdom., Jabbari S; University of Birmingham, School of Mathematics, Birmingham, West Midlands, B15 2TT, United Kingdom., Ward J; Loughborough University, Department of Mathematical Sciences, Epinal Way, Loughborough, Leicestershire, LE11 3TU, United Kingdom., Kuehne SA; University of Birmingham, School of Dentistry, 5 Mill Pool Way, Birmingham, West Midlands, B5 7EG, United Kingdom. |
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Jazyk: | angličtina |
Zdroj: | Biofilm [Biofilm] 2023 May 10; Vol. 5, pp. 100115. Date of Electronic Publication: 2023 May 10 (Print Publication: 2023). |
DOI: | 10.1016/j.bioflm.2023.100115 |
Abstrakt: | Chronic wounds are a drain on global health services and remain a major area of unmet clinical need. Chronic wounds are characterised by a stable and stubborn bacterial biofilm which hinders innate immune response and delays or prevents wound healing. Bioactive glass (BG) fibres offer a promising novel treatment for chronic wounds by targeting the wound-associated biofilm. In this study, the antimicrobial properties of silver-doped BG fibres were tested against Pseudomonas aeruginosa biofilms, which are commonly found in chronic wound infections. Results showed that BG fibres doped with silver resulted in a 5log10 reduction in biofilm formation whereas silver-free fibres only reduced formation by log10, therefore silver-doped fibres possess stronger antimicrobial effects. Moreover, there appeared to be a synergistic effect between the fibres and the silver as the application of the silver-doped fibres placed directly in contact with the forming biofilm resulted in a higher reduction in biofilm formation compared to treatments either: using the dissolution ions, using BG powder, or when the fibres were placed in an insert above the biofilm, inhibiting physical contact, instead. This suggests that the physical properties of the fibres, as well as silver, influence biofilm formation. Finally, results demonstrated that silver chloride, which is not antimicrobial, forms and the concentrations of antimicrobial silver species, namely silver ions and nanoparticles, reduce over time when fibres are soaked in cell culture media, which partially explains why the silver-doped dissolution ions contained lower antimicrobial activity compared to the fibres. As silver chloride is more likely to form with increased temperature and time, the antimicrobial activity of silver-containing dissolution ions is highly dependent on the length of ageing and storage conditions. Many studies investigate the antimicrobial and cytotoxic properties of biomaterials through their dissolution products. However, instability of antimicrobial silver species due to silver chloride formation and its effect on antimicrobial properties of silver-based biomaterials has not been reported before and could influence past and future dissolution-based assays as results showed that the antimicrobial activity of silver-based dissolution ions can vary greatly depending on post processing steps and can therefore produce misleading data. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Sandeep Shirgill reports financial support was provided by 10.13039/501100000268Biotechnology and Biological Sciences Research Council. Gowsihan Poologasundarampillai reports financial support was provided by 10.13039/501100000266Engineering and Physical Sciences Research Council. (© 2023 The Authors.) |
Databáze: | MEDLINE |
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