Brain-derived neurotrophic factor contributes to neurogenesis after intracerebral hemorrhage: a rodent model and human study.

Autor:	Lin TC; Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan., Tsai YC; Department of Neurology and Stroke Center, National Taiwan University Hospital, Taipei, Taiwan., Chen YA; Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan., Young TH; Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan., Wu CC; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.; Department of Neurosurgery, Taipei Medical University Hospital, Taipei, Taiwan.; TMU Neuroscience Research Center, Taipei Medical University, Taipei, Taiwan.; Taipei Neuroscience Institute, Taipei Medical University, Taipei, Taiwan., Chiang YH; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.; Department of Neurosurgery, Taipei Medical University Hospital, Taipei, Taiwan.; TMU Neuroscience Research Center, Taipei Medical University, Taipei, Taiwan.; Taipei Neuroscience Institute, Taipei Medical University, Taipei, Taiwan., Kao CH; Department of Neurology and Stroke Center, National Taiwan University Hospital, Taipei, Taiwan., Huang AP; Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan., Hsu YH; Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan., Chen KY; TMU Neuroscience Research Center, Taipei Medical University, Taipei, Taiwan.; Ph.D. Program in Medical Neuroscience, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan., Tsai LK; Department of Neurology and Stroke Center, National Taiwan University Hospital, Taipei, Taiwan.; Department of Neurology, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, Taiwan.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular neuroscience [Front Cell Neurosci] 2023 May 11; Vol. 17, pp. 1170251. Date of Electronic Publication: 2023 May 11 (Print Publication: 2023).
DOI:	10.3389/fncel.2023.1170251
Abstrakt:	Background and Purpose: Intracerebral hemorrhage (ICH) enhances neurogenesis in the subventricular zone (SVZ); however, the mechanism is not fully understood. We investigated the role of brain-derived neurotrophic factor (BDNF) in post-ICH neurogenesis in a rodent model and in patients with ICH using cerebrospinal fluid (CSF). Methods: A rat model of ICH was constructed via stereotaxic injection of collagenase into the left striatum. Patients with ICH receiving an external ventricular drain were prospectively enrolled. CSF was collected from rats and patients at different post-ICH times. Primary cultured rat neural stem cells (NSCs) were treated with CSF with or without BDNF-neutralized antibody. Immunohistochemistry and immunocytochemistry were used to detect NSC proliferation and differentiation. The BDNF concentration in CSF was quantified using enzyme-linked immunosorbent assays (ELISA). Results: In the rat model of ICH, the percentage of proliferating NSCs and neuroblasts in SVZ was elevated in bilateral hemispheres. The cultured rat NSCs treated with CSF from both rats and patients showed an increased capacity for proliferation and differentiation toward neuroblasts. BDNF concentration was higher in CSF collected from rats and patients with ICH than in controls. Blocking BDNF decreased the above-noted promotion of proliferation and differentiation of cultured NSCs by CSF treatment. In patients with ICH, the BDNF concentration in CSF and the neurogenesis-promoting capacity of post-ICH CSF correlated positively with ICH volume. Conclusion: BDNF in CSF contributes to post-ICH neurogenesis, including NSC proliferation and differentiation toward neuroblasts in a rat model and patients with ICH. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Lin, Tsai, Chen, Young, Wu, Chiang, Kao, Huang, Hsu, Chen and Tsai.)
Databáze:	MEDLINE
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