Green-synthesized nanoparticles of the polyherbal extract attenuate the necrosis of pancreatic β-cell in a streptozotocin-induced diabetic model.

Autor:	Hasan Chowdhury MA; Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh., Al Araby SQ; Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh., Alelwani W; Department of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia., Kattan SW; Medical Laboratory Department, College of Applied Medical Sciences, Taibah University, Yanbu, Saudi Arabia., Mansouri OA; Department of Biology, Collage of Science, University of Jeddah, Saudi Arabia., Uddin Rahat MR; Department of Pharmacy, International Islamic University Chittagong, Kumira, Sitakunda, Chittagong-4318, Bangladesh., Khan M; Bangladesh Reference Institute for Chemical Measurements (BRiCM), Dr. Qudrat-i-Khuda Road, Dhanmondi, Dhaka-1205, Bangladesh., Tangpong J; School of Allied Health Sciences, Walailak University, Nakkhon-Shi Thammarat, Nakhon Si Thammarat, 80160, Thailand., Rahman MA; Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh.; School of Allied Health Sciences, Walailak University, Nakkhon-Shi Thammarat, Nakhon Si Thammarat, 80160, Thailand.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 May 15; Vol. 9 (5), pp. e16137. Date of Electronic Publication: 2023 May 15 (Print Publication: 2023).
DOI:	10.1016/j.heliyon.2023.e16137
Abstrakt:	Plant-based nanoformulation is one of the novel approaches for therapeutic benefits. This research synthesized a silver nanoparticle from the polyherbal combination of four plants/seeds ( Momordica charantia , Trigonella foenum -graecum, Nigella sativa , and Ocimum sanctum ) and investigated its antidiabetic effects in streptozotocin-induced Wistar albino rat model. The polyherbal extract (PH) was extracted by the Soxhlet-solvent extraction method and the resulting crude extract was undergone for silver nanoparticle synthesis. The PH extract was subjected to a four-week intervention in fructose-fed streptozotocin-induced Wistar Albino rats' models and in vitro antioxidative tests. Experimental animals (age: 6-7 weeks, male, body weight: 200-220 g), were divided into five groups including normal control (NC), reference control (RC), diabetic control (DC), and treatment groups PH200, PH100, and PHAgNP20. After three weeks of intervention, body weight, weekly blood glucose level, oral glucose tolerance test, AST, ALT, alkaline phosphatase, total cholesterol, triglycerides, uric acid, urea, and creatinine level of PH200 were found to be significantly (P < 0.05) improved compared to the diabetic control. The same dose demonstrated better regeneration of damaged pancreatic and kidney tissues. In vitro antioxidant assay manifested promising IC50 values of 86.17 μg/mL for DPPH, 711.04 μg/mL for superoxide free radical, and 0.48 mg/mL for Iron chelating activity of the polyherbal extract. GC-MS analysis impacted the major volatile compounds of the PH. The data demonstrate that the PH and its nanoparticles could be a novel source of antidiabetic therapeutics through an advanced dose-response study in the type 2 diabetic model. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.)
Databáze:	MEDLINE
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