Evaluation of Dentin Remineralization with Zinc Oxide and Calcium Fluoride Nanoparticles - An In vitro Study.

Autor: Ahalya P; Department of Pedodontics and Preventive Dentistry, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India., Uloopi KS; Department of Pedodontics and Preventive Dentistry, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India., Vinay C; Department of Pedodontics and Preventive Dentistry, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India., RojaRamya KS; Department of Pedodontics and Preventive Dentistry, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India., Alla R; Department of Dental Materials, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India., RangaRaju P; Department of Oral Pathology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.
Jazyk: angličtina
Zdroj: Contemporary clinical dentistry [Contemp Clin Dent] 2023 Jan-Mar; Vol. 14 (1), pp. 57-61. Date of Electronic Publication: 2022 Nov 03.
DOI: 10.4103/ccd.ccd_701_21
Abstrakt: Background: Partially demineralized dentin is remineralizable when mineral ions are made available in the near vicinity. Nanoparticles (NPs) have wide applications in remineralization process. Zinc promotes remineralization and has a synergistic effect when combined with fluoride. Hence, zinc oxide and calcium fluoride NPs were considered for dentin remineralization.
Aim: The aim of this study was to evaluate the remineralizing ability of zinc oxide and calcium fluoride NPs on demineralized dentin individually and in combined form.
Settings and Design: This was an in vitro study.
Materials and Methods: Forty-eight dentin disks were prepared from the crowns of 12 extracted human molars and were allocated into four groups of 12 each, i.e., Group I - nano zinc oxide (nZnO), Group II - nano calcium fluoride (nCaF 2 ), Group III - combined group (nZnO + nCaF 2 ), and Group IV - artificial saliva (AS, control group). The specimens were demineralized with 37% phosphoric acid and placed in the respective remineralizing solutions for 24 h and 1 month. Calcium (Ca) and phosphorous (P) uptake was measured using energy-dispersive X-ray spectrometry, and structural changes were analyzed using scanning electron microscopy (SEM).
Statistical Analysis: One-way analysis of variance, Student's t -test, and post hoc Tukey's test were used for statistical analysis.
Results: At 1-month interval, all the groups showed an increase in Ca/P ratio, with highest being the combined group (4.24), followed by nCaF 2 (3.30), nZnO (1.71), and AS (1.31) groups, and these differences were statistically significant ( P = 0.000). On SEM analysis of dentinal samples at 1 month, depositions were evident in intertubular regions, wherein the highest deposits were observed in the nZnO group, followed by nZnO + nCaF 2 and nCaF 2 groups.
Conclusion: Dentin samples subjected to remineralization with aqueous solutions of nZnO and nCaF 2 showed an increase in calcium and phosphorous uptake and also dense granular depositions were evident in intertubular regions of dentin.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2022 Contemporary Clinical Dentistry.)
Databáze: MEDLINE
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