Separation and identification of different therapeutic pharmaceutical drugs on humic acid-based HPLC column: Method optimization using central composite design.

Autor: Ayyildiz HF; Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Selçuk University, Konya, Turkey., Memon FN; Department of Chemistry, University of Karachi, Karachi, Pakistan., Mangi IA; Department of Chemistry, University of Karachi, Karachi, Pakistan., Kara H; Department of Chemistry, Faculty of Science, Selçuk University, Konya, Turkey.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2023 Jul; Vol. 46 (14), pp. e2200914. Date of Electronic Publication: 2023 May 30.
DOI: 10.1002/jssc.202200914
Abstrakt: Multimode chromatographic separations are highly desirable in pharmaceutical and environmental sciences. Current study deals with the application of newly developed mixed-mode end capped-immobilized humic acid onto an aminopropyl silica based chromatographic column for separation and identification of six drugs belonging to different therapeutic groups for its applicability in pharmaceutical industries. For this, central composite design was used to evaluate the separation and resolution by optimization of three most effective parameters (acetonitrile%, flow rate, and pH of mobile phase). Second-order quadratic model was used to evaluate their effect on resolution of peaks; the probability value (<0.05) obtained from analysis of variance suggested the best applicability of the model. Desirability function was applied to calculate optimum conditions (44.8% acetonitrile, 1.75 mL/min of flow rate, and 7.5 pH) required to achieve maximum separation with good resolution within 11 min. The method was validated for linearity, precision accuracy, selectivity, and sensitivity. The results revealed a highly precise (coefficient of variance > 1%), linear (R 2  = 0.99), and highly selective method. Moreover, the limit of detection/quantification values revealed acceptable sensitivity of the method. The developed column was compared for its efficiency with a commercially available column and found to be highly applicable for industrial applications.
(© 2023 The Authors. Journal of Separation Science published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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