Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop-P-methyl resistance in Digitaria sanguinalis.

Autor: Liu X; College of Plant Protection, Hunan Agricultural University, Changsha, China.; Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha, China., Hou Z; College of Plant Protection, Hunan Agricultural University, Changsha, China., Zhang Y; College of Plant Protection, Hunan Agricultural University, Changsha, China., Merchant A; Department of Entomology, University of Kentucky, Lexington, KY, USA., Zhong ME; School of Chemistry and Materials Science, Hunan Agricultural University, Changsha, China., Ma G; Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha, China., Zeng Q; Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha, China., Wu L; Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha, China., Zhou X; Department of Entomology, University of Kentucky, Lexington, KY, USA., Luo K; College of Plant Protection, Hunan Agricultural University, Changsha, China., Ding C; College of Plant Protection, Hunan Agricultural University, Changsha, China.; School of Chemistry and Materials Science, Hunan Agricultural University, Changsha, China.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2023 Oct; Vol. 79 (10), pp. 3950-3958. Date of Electronic Publication: 2023 Jun 28.
DOI: 10.1002/ps.7588
Abstrakt: Background: Haloxyfop-P-methyl, an acetyl-CoA carboxylase (ACCase)-inhibiting herbicide, has been extensively used to control grass weeds. Widespread use of haloxyfop-P-methyl in cotton fields in China has led to the development of glutathione transferase (GST)-mediated resistance in Digitaria sanguinalis. An RNA-seq analysis identified DsGSTU1, a tau class glutathione transferase from the D. sanguinalis transcriptome as a potential candidate. Here, we cloned DsGSTU1 from D. sanguinalis young leaf tissues and subsequently characterized DsGSTU1 by a combination of sequence analysis, as well as functional heterologous expression in rice.
Results: The full-length coding DNA sequence (CDS) of DsGSTU1 is 717 bp in length. Higher DsGSTU1 expression was observed in haloxyfop-P-methyl-resistant (HR) D. sanguinalis than in haloxyfop-P-methyl-susceptible (HS) plants. Overexpression of the DsGSTU1 gene was confirmed by transformation into the wild-type (WT) Nipponbare rice with pBWA(V)HS, a recombinant expression vector. GST activity in transgenic rice seedlings was 1.18-1.40-fold higher than the WT rice seedlings before and after haloxyfop-P-methyl treatment, respectively. Additionally, transgenic rice seedlings overexpressing DsGSTU1 were less sensitive to haloxyfop-P-methyl.
Conclusion: Our combined findings suggest that DsGSTU1 is involved in metabolic resistance to haloxyfop-P-methyl in D. sanguinalis. A better understanding of the major genes contributing to herbicide-resistant D. sanguinalis facilitates the development of resistance management strategies for this global invasive grass weed. © 2023 Society of Chemical Industry.
(© 2023 Society of Chemical Industry.)
Databáze: MEDLINE
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