Anti-Toxoplasma gondii activity of Trametes versicolor (Turkey tail) mushroom extract.

Autor: Sharma HN; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.; Microbiology Ph.D. Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.; Laboratory of Ethnomedicine, Parasitology and Drug Discovery, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA., Catrett J; Enterprise State Community College, Enterprise, AL, USA., Nwokeocha OD; Department of Chemistry, College of Arts and Sciences, Tuskegee University, Tuskegee, AL, 36088, USA.; The School of Dentistry (SOD) Doctorate of Dentistry Program, Meharry Medical College, Nashville, TN, USA., Boersma M; Department of Chemistry and Biochemistry, College of Science and Mathematics (COSAM), Auburn University, Auburn, AL, 36849, USA., Miller ME; Auburn University Research Instrumentation Facility, Harrison College of Pharmacy, Auburn University, Auburn, AL, 36849, USA., Napier A; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.; Microbiology Ph.D. Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA., Robertson BK; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.; Microbiology Ph.D. Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA., Abugri DA; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA. dabugri@alasu.edu.; Microbiology Ph.D. Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA. dabugri@alasu.edu.; Laboratory of Ethnomedicine, Parasitology and Drug Discovery, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA. dabugri@alasu.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 29; Vol. 13 (1), pp. 8667. Date of Electronic Publication: 2023 May 29.
DOI: 10.1038/s41598-023-35676-6
Abstrakt: Toxoplasma gondii (T. gondii) infection continues to rise globally in humans and animals with high socioeconomic and public health challenges. Current medications used against T. gondii infection are limited in efficacy, safety, and affordability. This research was conducted to assess the higher fungi extract effect on T. gondii tachyzoites growth in vitro and possibly decipher its mechanism of action. Furthermore, we evaluated the extract's effect on human foreskin fibroblast viability. The methanol extracts of Turkey tail (TT) mushroom was tested against T. gondii tachyzoites growth using an RH-RFP type I strain that expresses red fluorescent protein throughout culture in a dose-dependent manner using a fluorescent plate reader. Similarly, we tested the effect of the extract on host cell viability. We observed that TT extract inhibited tachyzoites growth with a 50% minimum inhibitory concentration (IC 50s ), IC 50  = 5.98 ± 1.22 µg/mL, and 50% cytotoxic concentration (CC 50s ), CC 50  ≥ 100 µg/mL. It was discovered that TT extract induced strong mitochondria superoxide and  reactive oxygen species production and disrupted mitochondria membrane potential in T. gondii tachyzoites. Additionally, scanning electron microscopy depicted that TT extract and pyrimethamine (PY) caused a morphological deformation of tachyzoites in vitro. In conclusion, TT methanol extract made up of phytosterols, bioactive sphingolipids, peptides, phenolic acids, and lactones could be a promising source of new compounds for the future development of anti-Toxoplasma gondii drugs. Extracts were non-cytotoxic, even at higher concentrations.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje