Nanobody-based immunomagnetic separation platform for rapid isolation and detection of Salmonella enteritidis in food samples.

Autor: Bai M; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Wang Y; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Zhang C; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Wang Y; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Wei J; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Liao X; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Wang J; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China., Anfossi L; Department of Chemistry, University of Turin, Via Pietro Giuria 7, 10137 Turin, TO, Italy., Wang Y; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: yanruwang22@163.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2023 Oct 30; Vol. 424, pp. 136416. Date of Electronic Publication: 2023 May 19.
DOI: 10.1016/j.foodchem.2023.136416
Abstrakt: Rapid separation and identification of Salmonella enteritidis (S. enteritidis) in food is of great importance to prevent outbreaks of foodborne diseases. Herein, by using O and H antigens as targets, an epitope-based bio-panning strategy was applied to isolate specific nanobodies towards S. enteritidis. This method constitutes an efficient way to obtain specific antibody fragments and test pairwise nanobodies. On this basis, a double nanobody-based sandwich enzyme-linked immunosorbent assay (ELISA) coupled with immunomagnetic separation (IMS) was developed to rapid enrich and detect S. enteritidis in food. The detection limit of the IMS-ELISA was 3.2 × 10 3 CFU/mL. In addition, 1 CFU of S. enteritidis in food samples can be detected after 4-h cultivation, which was shortened by 2 h after IMS. The IMS-ELISA strategy could avoid matrix interference and shorten the enrichment culture time, which has great potential for application in monitoring bacterial contamination in food.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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