Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology.

Autor: Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Scudese E; Laboratory of Biosciences of Human Motricity (LABIMH) of the Federal University of State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil.; Sport Sciences and Exercise Laboratory (LaCEE), Catholic University of Petrópolis (UCP), Catholic, 25685-100, Brazil., Lopez EG; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Shao B; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Krystofiak E; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, 37232, USA., Rutledge S; Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL, 35899, USA., Davis J; Department of Biochemistry, Cancer Biology, Neuroscience, Pharmacology, Meharry Medical College, Nashville, TN, 37232, USA., Murray SA; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, 15261, USA., Damo SM; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37208, USA.; Center for Structural Biology, Vanderbilt University, Nashville, TN, 37232, USA., Katti P; National Heart, Lung and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA., Hinton A Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Jazyk: angličtina
Zdroj: Advanced biology [Adv Biol (Weinh)] 2023 Aug; Vol. 7 (8), pp. e2300139. Date of Electronic Publication: 2023 May 28.
DOI: 10.1002/adbi.202300139
Abstrakt: Serial block face scanning electron microscopy (SBF-SEM), also referred to as serial block-face electron microscopy, is an advanced ultrastructural imaging technique that enables three-dimensional visualization that provides largerx- and y-axis ranges than other volumetric EM techniques. While SEM is first introduced in the 1930s, SBF-SEM is developed as a novel method to resolve the 3D architecture of neuronal networks across large volumes with nanometer resolution by Denk and Horstmann in 2004. Here, the authors provide an accessible overview of the advantages and challenges associated with SBF-SEM. Beyond this, the applications of SBF-SEM in biochemical domains as well as potential future clinical applications are briefly reviewed. Finally, the alternative forms of artificial intelligence-based segmentation which may contribute to devising a feasible workflow involving SBF-SEM, are also considered.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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