Development of electrochemical Zn 2+ sensors for rapid voltammetric detection of glucose-stimulated insulin release from pancreatic β-cells.

Autor: Vanderlaan EL; Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN, USA; Indiana Medical Scientist/Engineer Training Program, Indiana University School of Medicine, Indianapolis, IN, USA., Nolan JK; Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN, USA; Center for Implantable Devices, Birck Nanotechnology Center, Purdue University, West Lafayette, IN, USA., Sexton J; Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN, USA., Evans-Molina C; Indiana Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA; Roudebush VA Medical Center, Indianapolis, IN, USA., Lee H; Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN, USA; Center for Implantable Devices, Birck Nanotechnology Center, Purdue University, West Lafayette, IN, USA., Voytik-Harbin SL; Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN, USA; Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA. Electronic address: harbins@purdue.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2023 Sep 01; Vol. 235, pp. 115409. Date of Electronic Publication: 2023 May 18.
DOI: 10.1016/j.bios.2023.115409
Abstrakt: Diabetes is a chronic disease characterized by elevated blood glucose levels resulting from absent or ineffective insulin release from pancreatic β-cells. β-cell function is routinely assessed in vitro using static or dynamic glucose-stimulated insulin secretion (GSIS) assays followed by insulin quantification via time-consuming, costly enzyme-linked immunosorbent assays (ELISA). In this study, we developed a highly sensitive electrochemical sensor for zinc (Zn 2+ ), an ion co-released with insulin, as a rapid and low-cost method for measuring dynamic insulin release. Different modifications to glassy carbon electrodes (GCE) were evaluated to develop a sensor that detects physiological Zn 2+ concentrations while operating within a biological Krebs Ringer Buffer (KRB) medium (pH 7.2). Electrodeposition of bismuth and indium improved Zn 2+ sensitivity and limit of detection (LOD), and a Nafion coating improved selectivity. Using anodic stripping voltammetry (ASV) with a pre-concentration time of 6 min, we achieved a LOD of 2.3 μg/L over the wide linear range of 2.5-500 μg/L Zn 2+ . Sensor performance improved with 10-min pre-concentration, resulting in increased sensitivity, lower LOD (0.18 μg/L), and a bilinear response over the range of 0.25-10 μg/L Zn 2+ . We further characterized the physicochemical properties of the Zn 2+ sensor using scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Finally, we demonstrated the sensor's capability to measure Zn 2+ release from glucose-stimulated INS-1 β-cells and primary mouse islets. Our results exhibited a high correlation with secreted insulin and validated the sensor's potential as a rapid alternative to conventional two-step GSIS plus ELISA methods.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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