Authentication of Platycladus Orientalis Leaves and Its Five Adulterants by Combination of Morphology and Microscopic Characteristics, TLC, and HPLC Analysis.

Autor:	Hao JH; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China., Xiao PT; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China., Duan PB; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China., Miao DY; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China., Jiang XY; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China., Kuang YJ; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China., Liu EH; China Pharmaceutical University, State Key Laboratory of Natural Medicines, No. 24 Tongjia Lane, Nanjing, PR China.
Jazyk:	angličtina
Zdroj:	Journal of AOAC International [J AOAC Int] 2023 Sep 01; Vol. 106 (5), pp. 1295-1304.
DOI:	10.1093/jaoacint/qsad066
Abstrakt:	Background: Platycladus orientalis leaves (POL), as the source of the traditional Chinese medicine (TCM) Platycladi Cacumen, has frequently been found to be misused with five adulterants including Chamaecyparis obtusa leaves (COL), Cupressus funebris leaves (CFL), Juniperus virginiana leaves (JVL), Sabina chinensis leaves (SCL), and Juniperus formosana leaves (JFL). Objective: The purpose of this study was to distinguish POL (fresh leaves) from its five adulterants (fresh leaves). Methods: The micromorphological features in terms of transection and microscopic characteristics of POL and adulterants were captured and compared using the an microscope. Both HPLC and TLC methods for the simultaneous determination of six bioactive flavonoids (myricitrin, isoquercitrin, quercitrin, amentoflavone, afzelin, and hinokiflavone) have been developed. Results: There were significant differences in microscopic features of transverse section and powders. The TLC results suggested that the spots of myricitrin in POL were more obvious than those in the five adulterants. The contents of myricitrin and quercitrin, or the total content of flavonoids in POL, determined by HPLC, were significantly higher than those in the adulterants. Conclusion: POL was successfully distinguished from its five adulterants by the comparison of morphology, microscopic characteristics, and chemical profiles. Highlights: This research provides a comprehensive morphology, microscopic identification, TLC, and HPLC analysis for authenticating POL and its five adulterants. (© The Author(s) 2023. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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