| Autor: |
da Rosa Pinheiro T; Graduate Program in Pharmaceutical Sciences, Center for Health Sciences, Department of Microbiology and Parasitology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., Dantas GA; Graduate Program in Pharmaceutical Sciences, Center for Health Sciences, Department of Microbiology and Parasitology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., da Silva JLG; Laboratory of Experimental and Applied Immunology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., Leal DBR; Laboratory of Experimental and Applied Immunology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., da Silva RB; Department of Physics, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., de Lima Burgo TA; Bioinorganic and Porphyrin Materials Laboratory, Department of Chemistry, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., Santos RCV; Graduate Program in Pharmaceutical Sciences, Center for Health Sciences, Department of Microbiology and Parasitology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil., Iglesias BA; Department of Chemistry and Environmental Sciences, Ibilce, São Paulo State University (Unesp), São Jose do Rio Preto 15054-000, Brazil. |
| Abstrakt: |
Onychomycosis is a prevalent nail fungal infection, and Candida albicans is one of the most common microorganisms associated with it. One alternative therapy to the conventional treatment of onychomycosis is antimicrobial photoinactivation. This study aimed to evaluate for the first time the in vitro activity of cationic porphyrins with platinum(II) complexes 4PtTPyP and 3PtTPyP against C. albicans . The minimum inhibitory concentration of porphyrins and reactive oxygen species was evaluated by broth microdilution. The yeast eradication time was evaluated using a time-kill assay, and a checkerboard assay assessed the synergism in combination with commercial treatments. In vitro biofilm formation and destruction were observed using the crystal violet technique. The morphology of the samples was evaluated by atomic force microscopy, and the MTT technique was used to evaluate the cytotoxicity of the studied porphyrins in keratinocyte and fibroblast cell lines. The porphyrin 3PtTPyP showed excellent in vitro antifungal activity against the tested C. albicans strains. After white-light irradiation, 3PtTPyP eradicated fungal growth in 30 and 60 min. The possible mechanism of action was mixed by ROS generation, and the combined treatment with commercial drugs was indifferent. The 3PtTPyP significantly reduced the preformed biofilm in vitro. Lastly, the atomic force microscopy showed cellular damage in the tested samples, and 3PtTPyP did not show cytotoxicity against the tested cell lines. We conclude that 3PtTPyP is an excellent photosensitizer with promising in vitro results against C. albicans strains. |
