| Autor: |
Dias BP; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Barbosa CC; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Ferreira CS; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Mayra Soares Alves Dos Santos S; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Arrieta OAP; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Malta WC; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Gomes MLMD; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Alves E Silva M; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Fonseca JM; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil., Borges LP; Department of Pharmacy, Federal University of Sergipe, São Cristóvão 9100-000, Brazil., Silva BM; Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, Brazil. |
| Abstrakt: |
Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology. |
