| Autor: |
Karaffová V; Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia., Teleky J; Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia., Pintarič M; Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, Slovenia., Langerholc T; Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, Slovenia., Mudroňová D; Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia., Hudec E; Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia., Ševčíková Z; Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia. |
| Abstrakt: |
In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri ( L. reuteri ), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 × 10 7 and 1 × 10 9 CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1β, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 × 10 5 cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 10 9 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation. |
