Exploring FeLV-Gag-Based VLPs as a New Vaccine Platform-Analysis of Production and Immunogenicity.

Autor: Ortiz R; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain.; Doctorate School, Microbiology Department, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Barajas A; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain.; Doctorate School, Medicine Department, University of Vic-Central University of Catalonia (UVic-UCC), 08500 Vic, Spain., Pons-Grífols A; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain.; Doctorate School, Microbiology Department, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Trinité B; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain., Tarrés-Freixas F; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain., Rovirosa C; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain., Urrea V; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain., Barreiro A; HIPRA, 17170 Amer, Spain., Gonzalez-Tendero A; HIPRA, 17170 Amer, Spain., Cardona M; HIPRA, 17170 Amer, Spain., Ferrer L; HIPRA, 17170 Amer, Spain., Clotet B; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain.; Doctorate School, Medicine Department, University of Vic-Central University of Catalonia (UVic-UCC), 08500 Vic, Spain.; Infectious Diseases Department, Germans Trias I Pujol Hospital, 08916 Badalona, Spain., Carrillo J; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain.; CIBERINFEC, ISCIII, 28029 Madrid, Spain., Aguilar-Gurrieri C; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain., Blanco J; AIDS Research Institute, IrsiCaixa, Campus Can Ruti, 08916 Badalona, Spain.; Doctorate School, Microbiology Department, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Doctorate School, Medicine Department, University of Vic-Central University of Catalonia (UVic-UCC), 08500 Vic, Spain.; CIBERINFEC, ISCIII, 28029 Madrid, Spain.; Germans Trias I Pujol Research Institute (IGTP), Campus Can Ruti, 08916 Badalona, Spain.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 May 19; Vol. 24 (10). Date of Electronic Publication: 2023 May 19.
DOI: 10.3390/ijms24109025
Abstrakt: Feline leukemia virus (FeLV) is one of the most prevalent infectious diseases in domestic cats. Although different commercial vaccines are available, none of them provides full protection. Thus, efforts to design a more efficient vaccine are needed. Our group has successfully engineered HIV-1 Gag-based VLPs that induce a potent and functional immune response against the HIV-1 transmembrane protein gp41. Here, we propose to use this concept to generate FeLV-Gag-based VLPs as a novel vaccine strategy against this retrovirus. By analogy to our HIV-1 platform, a fragment of the FeLV transmembrane p15E protein was exposed on FeLV-Gag-based VLPs. After optimization of Gag sequences, the immunogenicity of the selected candidates was evaluated in C57BL/6 and BALB/c mice, showing strong cellular and humoral responses to Gag but failing to generate anti-p15E antibodies. Altogether, this study not only tests the versatility of the enveloped VLP-based vaccine platform but also sheds light on FeLV vaccine research.
Databáze: MEDLINE
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