| Autor: |
Ullah A; Department of Health and Biological Sciences, Abasyn University, Peshawar 25000, Pakistan.; Institute for Pathology, University of Cologne, 50923 Cologne, Germany., Rehman IU; Center of Biotechnology and Microbiology, University of Peshawar, Peshawar 25000, Pakistan., Ommer K; Institute of Transfusion Medicine, University of Cologne, 50923 Cologne, Germany., Ahmed N; Center of Excellence in Molecular Biology, University of the Punjab, Lahore 54000, Pakistan., Odenthal M; Institute for Pathology, University of Cologne, 50923 Cologne, Germany., Yu X; Institute for Pathology, University of Cologne, 50923 Cologne, Germany., Ahmad J; Center of Biotechnology and Microbiology, University of Peshawar, Peshawar 25000, Pakistan., Nadeem T; Center of Excellence in Molecular Biology, University of the Punjab, Lahore 54000, Pakistan., Ali Q; Department of Plant Breeding and Genetics, University of the Punjab, Lahore 54000, Pakistan., Ahmad B; Center of Biotechnology and Microbiology, University of Peshawar, Peshawar 25000, Pakistan. |
| Abstrakt: |
MicroRNAs miR-29a and miR-192 are involved in inflammatory and fibrotic processes of chronic liver disease, and circulating miR-29a is suggested to diagnose fibrosis progression due to hepatitis C virus (HCV) infection. This study aimed to evaluate the expression profile of circulating miR-192 and 29a in a patient cohort with a high frequency of HCV genotype-3. A total of 222 HCV blood samples were collected and serum were separated. Patients were classified into mild, moderate, and severe liver injury based on their Child-Turcotte-Pugh CTP score. RNA was isolated from the serum and used for quantitative real-time PCR. The HCV genotype-3 (62%) was the predominant HCV genotype. In HCV patients, the serum miR-192 and miR-29a levels were significantly upregulated in comparison to healthy controls ( p = 0.0017 and p = 0.0001, respectively). The progression rate of miR-192 and 29a in the patient group with mild was highly upregulated compared to patients with moderate and severe hepatitis infection. The ROC curve of miR-192 and miR-29a of moderate liver disease had a significant diagnostic performance compared to the other HCV-infected groups. The increase in miR-29a and miR-192 serum levels was even slightly higher in patients with HCV genotype-3 than in non-genotype-3 patients. In conclusion, serum miR-192 and miR-29a levels significantly increased during the progression of chronic HCV infection. The marked upregulation in patients with HCV genotype-3 suggests them as potential biomarkers for hepatic disease, independently of the HCV genotype. |
