| Autor: |
Zenobi E; Hypatia Research Consortium, Via del Politecnico snc, 00133 Rome, Italy.; E. Amaldi Foundation, Via del Politecnico snc, 00133 Rome, Italy., Merco M; Institute of Translational Pharmacology, National Research Council, Via Fosso del Cavaliere 100, 00133 Rome, Italy., Mochi F; Hypatia Research Consortium, Via del Politecnico snc, 00133 Rome, Italy., Ruspi J; Biomedical Engineering, Department of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Piazzale Aldo Moro, 00184 Rome, Italy., Pecci R; National Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, Viale Regina Elena, 00161 Rome, Italy., Marchese R; Department of Clinical Pathology, Fatebenefratelli S. Peter Hospital, Via Cassia, 00189 Rome, Italy., Convertino A; Institute for Microelectronics and Microsystems, National Research Council, Via Fosso del Cavaliere 100, 00133 Rome, Italy., Lisi A; Institute of Translational Pharmacology, National Research Council, Via Fosso del Cavaliere 100, 00133 Rome, Italy., Del Gaudio C; Italian Space Agency, Via del Politecnico snc, 00133 Rome, Italy., Ledda M; Institute of Translational Pharmacology, National Research Council, Via Fosso del Cavaliere 100, 00133 Rome, Italy. |
| Abstrakt: |
Material extrusion (MEX), commonly referred to as fused deposition modeling (FDM) or fused filament fabrication (FFF), is a versatile and cost-effective technique to fabricate suitable scaffolds for tissue engineering. Driven by a computer-aided design input, specific patterns can be easily collected in an extremely reproducible and repeatable process. Referring to possible skeletal affections, 3D-printed scaffolds can support tissue regeneration of large bone defects with complex geometries, an open major clinical challenge. In this study, polylactic acid scaffolds were printed resembling trabecular bone microarchitecture in order to deal with morphologically biomimetic features to potentially enhance the biological outcome. Three models with different pore sizes (i.e., 500, 600, and 700 µm) were prepared and evaluated by means of micro-computed tomography. The biological assessment was carried out seeding SAOS-2 cells, a bone-like cell model, on the scaffolds, which showed excellent biocompatibility, bioactivity, and osteoinductivity. The model with larger pores, characterized by improved osteoconductive properties and protein adsorption rate, was further investigated as a potential platform for bone-tissue engineering, evaluating the paracrine activity of human mesenchymal stem cells. The reported findings demonstrate that the designed microarchitecture, better mimicking the natural bone extracellular matrix, favors a greater bioactivity and can be thus regarded as an interesting option for bone-tissue engineering. |
