| Autor: |
Turco G; Calico Life Sciences LLC, South San Francisco, CA, USA., Chang C; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Wang RY; Calico Life Sciences LLC, South San Francisco, CA, USA., Kim G; Calico Life Sciences LLC, South San Francisco, CA, USA., Stoops EH; Calico Life Sciences LLC, South San Francisco, CA, USA., Richardson B; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Sochat V; Lawrence Livermore National Laboratory, Livermore, CA, USA., Rust J; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Oughtred R; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Thayer N; Calico Life Sciences LLC, South San Francisco, CA, USA., Kang F; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Livstone MS; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Heinicke S; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Schroeder M; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Dolinski KJ; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA., Botstein D; Calico Life Sciences LLC, South San Francisco, CA, USA., Baryshnikova A; Calico Life Sciences LLC, South San Francisco, CA, USA. |
| Abstrakt: |
Genome-wide phenotypic screens in the budding yeast Saccharomyces cerevisiae , enabled by its knockout collection, have produced the largest, richest, and most systematic phenotypic description of any organism. However, integrative analyses of this rich data source have been virtually impossible because of the lack of a central data repository and consistent metadata annotations. Here, we describe the aggregation, harmonization, and analysis of ~14,500 yeast knockout screens, which we call Yeast Phenome. Using this unique dataset, we characterized two unknown genes ( YHR045W and YGL117W ) and showed that tryptophan starvation is a by-product of many chemical treatments. Furthermore, we uncovered an exponential relationship between phenotypic similarity and intergenic distance, which suggests that gene positions in both yeast and human genomes are optimized for function. |
