Blood-brain barrier remodeling in an organ-on-a-chip device shows Dkk1 to be a regulator of early metastasis.

Autor: Westerhof TM; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Yang BA; School of Engineering, Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Merill NM; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Yates JA; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Altemus M; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Russell L; School of Engineering, Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Miller AJ; School of Engineering, Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Bao L; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Wu Z; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Ulintz PJ; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Aguilar CA; School of Engineering, Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Morikawa A; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Castro MG; Michigan Medicine, Department of Neurosurgery, University of Michigan, Ann Arbor, MI 48109, USA.; Michigan Medicine, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA., Merajver SD; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Oliver CR; Michigan Medicine, Department of Internal Medicine, Division of Hematology/Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Jazyk: angličtina
Zdroj: Advanced nanobiomed research [Adv Nanobiomed Res] 2023 Apr; Vol. 3 (4). Date of Electronic Publication: 2023 Feb 05.
DOI: 10.1002/anbr.202200036
Abstrakt: Brain metastases are the most lethal progression event, in part because the biological processes underpinning brain metastases are poorly understood. There is a paucity of realistic models of metastasis, as current in vivo murine models are slow to manifest metastasis. We set out to delineate metabolic and secretory modulators of brain metastases by utilizing two models consisting of in vitro microfluidic devices: 1) a blood brain niche (BBN) chip that recapitulates the blood-brain-barrier and niche; and 2) a migration chip that assesses cell migration. We report secretory cues provided by the brain niche that attract metastatic cancer cells to colonize the brain niche region. Astrocytic Dkk-1 is increased in response to brain-seeking breast cancer cells and stimulates cancer cell migration. Brain-metastatic cancer cells under Dkk-1 stimulation increase gene expression of FGF-13 and PLCB1. Further, extracellular Dkk-1 modulates cancer cell migration upon entering the brain niche.
Databáze: MEDLINE
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