| Autor: |
Leelavathi DE, Soffer EF, Dressler DE, Knowles J |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical sciences [J Pharm Sci] 1986 Apr; Vol. 75 (4), pp. 421-3. |
| DOI: |
10.1002/jps.2600750423 |
| Abstrakt: |
An automated high-performance liquid chromatographic method with electrochemical detection is described for the determination of indoramin, a new antihypertensive drug. The procedure involves a single extraction of the drug from alkaline plasma with chlorobutane. The chlorobutane extract is evaporated to dryness, reconstituted in methanol and injected into the chromatograph. Separation is achieved using a CN-bonded silica column and an isocratic elution with 0.01 M sodium phosphate in 50% acetonitrile. Detection is at 0.95 V applied potential on a glassy carbon electrode versus an Ag/AgCl electrode. Electrochemical methods that depend on a high oxidation potential suffer from rapid electrode passivation by significant amounts of impurities extracted from plasma. Therefore, the instrumentation includes a valve-switching unit to divert most of the impurities away from the electrode compartment; thus, maintaining sensitivity during the automated analysis of a large number of samples. An RSD of 5-15% was obtained in the concentration range of 0.5-100 ng/mL plasma. The limit of detection is 0.5 ng/mL. The method has been successfully used for the determination of indoramin in plasma samples from human subjects given a 50-mg oral dose of the drug. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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