Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides .

Autor: Aitouguinane M; Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech, URL-CNRST 05), Faculté des Sciences et Techniques, Université Cadi Ayyad, Marrakech 40000, Morocco.; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France., El Alaoui-Talibi Z; Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech, URL-CNRST 05), Faculté des Sciences et Techniques, Université Cadi Ayyad, Marrakech 40000, Morocco., Rchid H; Laboratoire de Biotechnologies et Valorisation des Ressources Végétales, Faculté des Sciences, Université Chouaib Doukkali, El Jadida 24000, Morocco., Fendri I; Laboratoire de Biotechnologie des Plantes Appliquée à l'Amélioration des Cultures, Faculté des Sciences de Sfax, Université de Sfax, Sfax 3000, Tunisia., Abdelkafi S; Laboratoire de Génie Enzymatique et de Microbiologie, Equipe de Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax 3000, Tunisia., El-Hadj MDO; Laboratoire de Protection des Ecosystèmes en Zones Arides et Semi-Arides, Faculté des Sciences de la Nature et de la vie BP 511, Université Kasdi Merbah de Ouargla, Ouargla 30000, Algeria., Boual Z; Laboratoire de Protection des Ecosystèmes en Zones Arides et Semi-Arides, Faculté des Sciences de la Nature et de la vie BP 511, Université Kasdi Merbah de Ouargla, Ouargla 30000, Algeria., Le Cerf D; Polymères Biopolymères Surfaces, Normandie Université, UNIROUEN, INSA Rouen, CNRS, UMR6270, F-76821 Mont Saint-Aignan, France., Rihouey C; Polymères Biopolymères Surfaces, Normandie Université, UNIROUEN, INSA Rouen, CNRS, UMR6270, F-76821 Mont Saint-Aignan, France., Gardarin C; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France., Dubessay P; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France., Michaud P; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France., Pierre G; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France., Delattre C; Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France.; Institut Universitaire de France (IUF), 1 Rue Descartes, F-75005 Paris, France., El Modafar C; Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech, URL-CNRST 05), Faculté des Sciences et Techniques, Université Cadi Ayyad, Marrakech 40000, Morocco.
Jazyk: angličtina
Zdroj: Marine drugs [Mar Drugs] 2023 May 16; Vol. 21 (5). Date of Electronic Publication: 2023 May 16.
DOI: 10.3390/md21050301
Abstrakt: Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides , respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (M w ) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants.
Databáze: MEDLINE
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