Sequencing by avidity enables high accuracy with low reagent consumption.

Autor: Arslan S; Element Biosciences, San Diego, CA, USA., Garcia FJ; Element Biosciences, San Diego, CA, USA., Guo M; Element Biosciences, San Diego, CA, USA., Kellinger MW; Element Biosciences, San Diego, CA, USA., Kruglyak S; Element Biosciences, San Diego, CA, USA., LeVieux JA; Element Biosciences, San Diego, CA, USA., Mah AH; Element Biosciences, San Diego, CA, USA., Wang H; Element Biosciences, San Diego, CA, USA., Zhao J; Element Biosciences, San Diego, CA, USA., Zhou C; Element Biosciences, San Diego, CA, USA., Altomare A; Element Biosciences, San Diego, CA, USA., Bailey J; Element Biosciences, San Diego, CA, USA., Byrne MB; Element Biosciences, San Diego, CA, USA., Chang C; Element Biosciences, San Diego, CA, USA., Chen SX; Element Biosciences, San Diego, CA, USA., Cho B; Element Biosciences, San Diego, CA, USA., Dennler CN; Element Biosciences, San Diego, CA, USA., Dien VT; Element Biosciences, San Diego, CA, USA., Fuller D; Element Biosciences, San Diego, CA, USA., Kelley R; Element Biosciences, San Diego, CA, USA., Khandan O; Element Biosciences, San Diego, CA, USA., Klein MG; Element Biosciences, San Diego, CA, USA., Kim M; Element Biosciences, San Diego, CA, USA., Lajoie BR; Element Biosciences, San Diego, CA, USA., Lin B; Element Biosciences, San Diego, CA, USA., Liu Y; Element Biosciences, San Diego, CA, USA., Lopez T; Element Biosciences, San Diego, CA, USA., Mains PT; Element Biosciences, San Diego, CA, USA., Price AD; Element Biosciences, San Diego, CA, USA., Robertson SR; Element Biosciences, San Diego, CA, USA., Taylor-Weiner H; Element Biosciences, San Diego, CA, USA., Tippana R; Element Biosciences, San Diego, CA, USA., Tomaney AB; Element Biosciences, San Diego, CA, USA., Zhang S; Element Biosciences, San Diego, CA, USA., Abtahi M; Element Biosciences, San Diego, CA, USA., Ambroso MR; Element Biosciences, San Diego, CA, USA., Bajari R; Element Biosciences, San Diego, CA, USA., Bellizzi AM; Element Biosciences, San Diego, CA, USA., Benitez CB; Element Biosciences, San Diego, CA, USA., Berard DR; Element Biosciences, San Diego, CA, USA., Berti L; Element Biosciences, San Diego, CA, USA., Blease KN; Element Biosciences, San Diego, CA, USA., Blum AP; Element Biosciences, San Diego, CA, USA., Boddicker AM; Element Biosciences, San Diego, CA, USA., Bondar L; Element Biosciences, San Diego, CA, USA., Brown C; Element Biosciences, San Diego, CA, USA., Bui CA; Element Biosciences, San Diego, CA, USA., Calleja-Aguirre J; Element Biosciences, San Diego, CA, USA., Cappa K; Element Biosciences, San Diego, CA, USA., Chan J; Element Biosciences, San Diego, CA, USA., Chang VW; Element Biosciences, San Diego, CA, USA., Charov K; Element Biosciences, San Diego, CA, USA., Chen X; Element Biosciences, San Diego, CA, USA., Constandse RM; Element Biosciences, San Diego, CA, USA., Damron W; Element Biosciences, San Diego, CA, USA., Dawood M; Element Biosciences, San Diego, CA, USA., DeBuono N; Element Biosciences, San Diego, CA, USA., Dimalanta JD; Element Biosciences, San Diego, CA, USA., Edoli L; Element Biosciences, San Diego, CA, USA., Elango K; Element Biosciences, San Diego, CA, USA., Faustino N; Element Biosciences, San Diego, CA, USA., Feng C; Element Biosciences, San Diego, CA, USA., Ferrari M; Element Biosciences, San Diego, CA, USA., Frankie K; Element Biosciences, San Diego, CA, USA., Fries A; Element Biosciences, San Diego, CA, USA., Galloway A; Element Biosciences, San Diego, CA, USA., Gavrila V; Element Biosciences, San Diego, CA, USA., Gemmen GJ; Element Biosciences, San Diego, CA, USA., Ghadiali J; Element Biosciences, San Diego, CA, USA., Ghorbani A; Element Biosciences, San Diego, CA, USA., Goddard LA; Element Biosciences, San Diego, CA, USA., Guetter AR; Element Biosciences, San Diego, CA, USA., Hendricks GL; Element Biosciences, San Diego, CA, USA., Hentschel J; Element Biosciences, San Diego, CA, USA., Honigfort DJ; Element Biosciences, San Diego, CA, USA., Hsieh YT; Element Biosciences, San Diego, CA, USA., Hwang Fu YH; Element Biosciences, San Diego, CA, USA., Im SK; Element Biosciences, San Diego, CA, USA., Jin C; Element Biosciences, San Diego, CA, USA., Kabu S; Element Biosciences, San Diego, CA, USA., Kincade DE; Element Biosciences, San Diego, CA, USA., Levy S; Element Biosciences, San Diego, CA, USA., Li Y; Element Biosciences, San Diego, CA, USA., Liang VK; Element Biosciences, San Diego, CA, USA., Light WH; Element Biosciences, San Diego, CA, USA., Lipsher JB; Element Biosciences, San Diego, CA, USA., Liu TL; Element Biosciences, San Diego, CA, USA., Long G; Element Biosciences, San Diego, CA, USA., Ma R; Element Biosciences, San Diego, CA, USA., Mailloux JM; Element Biosciences, San Diego, CA, USA., Mandla KA; Element Biosciences, San Diego, CA, USA., Martinez AR; Element Biosciences, San Diego, CA, USA., Mass M; Element Biosciences, San Diego, CA, USA., McKean DT; Element Biosciences, San Diego, CA, USA., Meron M; Element Biosciences, San Diego, CA, USA., Miller EA; Element Biosciences, San Diego, CA, USA., Moh CS; Element Biosciences, San Diego, CA, USA., Moore RK; Element Biosciences, San Diego, CA, USA., Moreno J; Element Biosciences, San Diego, CA, USA., Neysmith JM; Element Biosciences, San Diego, CA, USA., Niman CS; Element Biosciences, San Diego, CA, USA., Nunez JM; Element Biosciences, San Diego, CA, USA., Ojeda MT; Element Biosciences, San Diego, CA, USA., Ortiz SE; Element Biosciences, San Diego, CA, USA., Owens J; Element Biosciences, San Diego, CA, USA., Piland G; Element Biosciences, San Diego, CA, USA., Proctor DJ; Element Biosciences, San Diego, CA, USA., Purba JB; Element Biosciences, San Diego, CA, USA., Ray M; Element Biosciences, San Diego, CA, USA., Rong D; Element Biosciences, San Diego, CA, USA., Saade VM; Element Biosciences, San Diego, CA, USA., Saha S; Element Biosciences, San Diego, CA, USA., Tomas GS; Element Biosciences, San Diego, CA, USA., Scheidler N; Element Biosciences, San Diego, CA, USA., Sirajudeen LH; Element Biosciences, San Diego, CA, USA., Snow S; Element Biosciences, San Diego, CA, USA., Stengel G; Element Biosciences, San Diego, CA, USA., Stinson R; Element Biosciences, San Diego, CA, USA., Stone MJ; Element Biosciences, San Diego, CA, USA., Sundseth KJ; Element Biosciences, San Diego, CA, USA., Thai E; Element Biosciences, San Diego, CA, USA., Thompson CJ; Element Biosciences, San Diego, CA, USA., Tjioe M; Element Biosciences, San Diego, CA, USA., Trejo CL; Element Biosciences, San Diego, CA, USA., Trieger G; Element Biosciences, San Diego, CA, USA., Truong DN; Element Biosciences, San Diego, CA, USA., Tse B; Element Biosciences, San Diego, CA, USA., Voiles B; Element Biosciences, San Diego, CA, USA., Vuong H; Element Biosciences, San Diego, CA, USA., Wong JC; Element Biosciences, San Diego, CA, USA., Wu CT; Element Biosciences, San Diego, CA, USA., Yu H; Element Biosciences, San Diego, CA, USA., Yu Y; Element Biosciences, San Diego, CA, USA., Yu M; Element Biosciences, San Diego, CA, USA., Zhang X; Element Biosciences, San Diego, CA, USA., Zhao D; Element Biosciences, San Diego, CA, USA., Zheng G; Element Biosciences, San Diego, CA, USA., He M; Element Biosciences, San Diego, CA, USA., Previte M; Element Biosciences, San Diego, CA, USA. mprevite@elembio.com.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2024 Jan; Vol. 42 (1), pp. 132-138. Date of Electronic Publication: 2023 May 25.
DOI: 10.1038/s41587-023-01750-7
Abstrakt: We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer-nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. Avidity sequencing achieves high accuracy, with 96.2% and 85.4% of base calls having an average of one error per 1,000 and 10,000 base pairs, respectively. We show that the average error rate of avidity sequencing remained stable following a long homopolymer.
(© 2023. The Author(s).)
Databáze: MEDLINE
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