Synergistic effect of manganese (II) phosphate & diamond nanoparticles in electrochemical sensors for reactive oxygen species determination in seminal plasma.

Autor: Pérez E; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, c/ Francisco Tomás y Valiente, Nº7, Campus de Excelencia de la Universidad Autónoma de Madrid, 28049, Madrid, Spain., Vázquez L; Instituto de Ciencia de Materiales de Madrid (ICMM), CSIC, c/ Sor Juana Inés de la Cruz Nº3, Campus de Excelencia de la Universidad Autónoma de Madrid, 28049, Madrid, Spain., Quintana C; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, c/ Francisco Tomás y Valiente, Nº7, Campus de Excelencia de la Universidad Autónoma de Madrid, 28049, Madrid, Spain., Petit-Domínguez MD; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, c/ Francisco Tomás y Valiente, Nº7, Campus de Excelencia de la Universidad Autónoma de Madrid, 28049, Madrid, Spain., Casero E; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, c/ Francisco Tomás y Valiente, Nº7, Campus de Excelencia de la Universidad Autónoma de Madrid, 28049, Madrid, Spain., Blanco E; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, c/ Francisco Tomás y Valiente, Nº7, Campus de Excelencia de la Universidad Autónoma de Madrid, 28049, Madrid, Spain. Electronic address: elias.blanco@uam.es.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2023 Jul 11; Vol. 1264, pp. 341301. Date of Electronic Publication: 2023 Apr 29.
DOI: 10.1016/j.aca.2023.341301
Abstrakt: In this work, we explore the ability of manganese (II) phosphate (MnP) as a catalytic element for the determination of reactive oxygen species (ROS) in seminal plasma, when MnP is employed as modifier of a glassy carbon electrode. The electrochemical response of the manganese (II) phosphate-modified electrode shows a wave at around +0.65 V due to the oxidation of Mn 2+ to MnO 2 + , which is clearly enhanced after addition of superoxide, the molecule considered as the mother of ROS. Once proved the suitability of manganese (II) phosphate as catalyst, we evaluate the effect of including a 0D (diamond nanoparticles) or a 2D (ReS 2 ) nanomaterial in the sensor design. The system consisting of manganese (II) phosphate and diamond nanoparticles yielded the largest improvement of the response. The morphological characterization of the sensor surface was performed by scanning electron microscopy and atomic force microscopy, while cyclic and differential pulse voltammetry were employed for the electrochemical characterization of the sensor. After optimizing the sensor construction, calibration procedures by chronoamperometry were performed, leading to a linear relation between peak intensity and the superoxide concentration in the range of 1.1 10 -4  M - 1.0 10 -3  M with a limit of detection of 3.2 10 -5  M. Seminal plasma samples were analysed by the standard addition method. Moreover, the analysis of samples fortified with superoxide at the μM level leads to recoveries of 95%.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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