Changes in Protease-Activated Receptor and Trypsin-1 Expression Are Involved in the Therapeutic Effect of Mg 2+ Supplementation in Type 2 Diabetes-Induced Gastric Injury in Male Adult Rats.

Autor: Mehranfard N; Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran., Rezazadeh H; Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Soltani N; Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Dastgerdi AH; Department of Preclinical Medicine, School of Medicine, Najafabad Branch, Islamic Azad University, Isfahan, Iran., Ghanbari Rad M; Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Ghasemi M; Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Jazyk: angličtina
Zdroj: Advances in pharmacological and pharmaceutical sciences [Adv Pharmacol Pharm Sci] 2023 May 16; Vol. 2023, pp. 5703718. Date of Electronic Publication: 2023 May 16 (Print Publication: 2023).
DOI: 10.1155/2023/5703718
Abstrakt: Purpose: Gastric inflammation is common and usually severe in patients with type 2 diabetes mellitus (T2DM). Evidence suggests protease-activated receptors (PARs) are a link between inflammation and gastrointestinal dysfunction. Given that magnesium (Mg 2+ ) deficiency is a highly prevalent condition in T2DM patients, we assessed the therapeutic role of Mg 2+ on the factors involved in gastric inflammation in T2DM.
Methods: A rat model of T2DM gastropathy was established using a long-term high-fat diet + a low dose of streptozocin. Twenty-four rats were divided into control, T2DM , T2DM + insulin (positive control), and T2DM + Mg 2+ groups. At the end of 2-month therapies, changes in the expression of gastric trypsin-1, PAR1, PAR2, PAR3, PI3K/Akt, and COX-2 proteins were measured by western blot. Hematoxylin and eosin and Masson's trichrome staining were used to detect gastric mucosal injury and fibrosis.
Results: The expression of trypsin-1, PAR1, PAR2, PAR3, and COX-2 increased in diabetes, and Mg 2+ /insulin treatment strongly decreased their expression. The PI3K/p-Akt significantly decreased in T2DM, and treatment with Mg 2+ /insulin improved PI3K in T2DM rats. Staining of the gastric antrum tissue of the insulin/Mg 2+ -treated T2DM rats showed a significantly minimal mucosal and fibrotic injury compared with those of rats from the T2DM group.
Conclusion: Mg 2+ supplement, comparable to insulin, via decreasing PARs expression, mitigating COX-2 activity, and decreasing collagen deposition could exert a potent gastroprotective effect against inflammation, ulcer, and fibrotic development in T2DM patients.
Competing Interests: The authors declare that there are no conflicts of interest.
(Copyright © 2023 Nasrin Mehranfard et al.)
Databáze: MEDLINE
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