A comparison of the chemiluminescence immunoassay and Crithidia luciliae immunofluorescence test in detecting anti-dsDNA antibodies and assessing the activity of systemic lupus erythematosus.
Autor: | He S; Department of Clinical Laboratory, Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China., Wu X; Department of Clinical Laboratory, Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China., Li L; Department of Clinical Laboratory, Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China., Jiang K; Department of Clinical Laboratory, Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China., He Q; Department of Clinical Laboratory, Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China., Xie L; Department of Clinical Laboratory, Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China. |
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Jazyk: | angličtina |
Zdroj: | Lupus [Lupus] 2023 Jul; Vol. 32 (8), pp. 936-941. Date of Electronic Publication: 2023 May 24. |
DOI: | 10.1177/09612033231179888 |
Abstrakt: | Objectives: This study aimed to compare the YHLO chemiluminescence immunoassay (CLIA) with the Crithidia luciliae immunofluorescence test (CLIFT) to detect anti-dsDNA antibodies and its correlation with disease activity in systemic lupus erythematosus (SLE). Method: In total, 208 patients diagnosed with SLE, 110 other autoimmune patients, 70 infectious disorders patients, and 105 healthy people were enrolled in this study. Serum samples were tested using CLIA in a YHLO chemiluminescence system and CLIFT. Results: The overall agreement between YHLO CLIA and CLIFT was 76.9% (160/208), with a moderate correlation (kappa = 0.530, p < 0.001). The sensitivity of YHLO CLIA and CLIFT were 58.2% and 55.3%, respectively. The specificity of YHLO CLIA and CLIFT were 95.1% and 99.3%, respectively. The sensitivity of YHLO CLIA was increased to 66.8% with a specificity of 93.6% when the cut-off value was set at 24 IU/mL. Spearman's correlation coefficient between the quantitative results of YHLO CLIA and the titers of CLIFT was 0.59 ( p < .01). A significant correlation was found between the anti-dsDNA results detected by YHLO CLIA and the SLE Disease Activity Index 2000 (SLEDAI-2K). Spearman's correlation coefficient between YHLO CLIA and SLEDAI-2K (r = 0.66, p < .01) was higher than that of CLIFT (r = 0.60, p < .01). Conclusions: Good correlation and agreement were found between YHLO CLIA and CLIFT. In addition, there was a significant correlation between YHLO CLIA and the SLE Disease Activity Index, which was superior to that of CLIFT. The YHLO chemiluminescence system is recommended for the assessment of disease activity. |
Databáze: | MEDLINE |
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