| Autor: |
Huq MA; Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea., Ma J; World Data Center for Microorganisms (WDCM); Institute of Microbiology, Chinese Academy of Sciences (IMCAS), PR China., Srinivasan S; Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Republic of Korea., Parvez MAK; Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh., Rahman MM; Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University, Kushtia-7003, Bangladesh., Naserkheil M; Animal Breeding and Genetics Division, National Institute of Animal Science, Cheonan 31000, Republic of Korea., Abuhena M; Department of Research & Development, Apex Biofertilizers & Biopesticides Limited, Gobindaganj-5740, Gaibandha, Bangladesh., Maitra P; Institute of Dendrology, Polish Academy of Sciences, 62-035 Kórnik, Poland., Islam F; Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Dhaka, Bangladesh., Nam K; Department of Horticultural Life Science, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea., Park JH; Department of Food Science and Biotechnology, Gachon University, Seongnam, 461-701, Republic of Korea., Akter S; Department of Food Science and Biotechnology, Gachon University, Seongnam, 461-701, Republic of Korea. |
| Abstrakt: |
A Gram-stain-negative, aerobic, short rod-shaped and motile novel bacterial strain, designated MAHUQ-52 T , was isolated from the rhizospheric soil of a banana plant. Colonies grew at 10-35 °C (optimum, 28 °C), pH 6.0-9.5 (optimum, pH 7.0-7.5), and in the presence of 0-1.0 % NaCl (optimum 0 %). The strain was positive for catalase and oxidase tests, as well as hydrolysis of gelatin, casein, starch and Tween 20. Based on the results of phylogenetic analysis using 16S rRNA gene and genome sequences, strain MAHUQ-52 T clustered together within the genus Massilia . Strain MAHUQ-52 T was closely related to Massilia soli R798 T (98.6 %) and Massilia polaris RP-1-19 T (98.3 %). The novel strain MAHUQ-52 T has a draft genome size of 4 677 454 bp (25 contigs), annotated with 4193 protein-coding genes, 64 tRNA and 19 rRNA genes. The genomic DNA G+C content was 63.0 %. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-52 T and closely related type strains were ≤88.4 and 35.8 %, respectively. The only respiratory quinone was ubiquinone-8. The major fatty acids were identified as C 16 : 0 and summed feature 3 (C 15 : 0 iso 2-OH and/or C 16 : 1 ω 7 c ). Strain MAHUQ-52 T contained phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol as the major polar lipids. On the basis of dDDH and ANI values, as well as genotypic, chemotaxonomic and physiological data, strain MAHUQ-52 T represents a novel species within the genus Massilia , for which the name Massilia agrisoli sp. nov. is proposed, with MAHUQ-52 T (=KACC 21999 T =CGMCC 1.18577 T ) as the type strain. |
