BODIPY-based near-infrared semiconducting polymer dot for selective yellow laser-excited cell imaging.

Autor:	Chen L; Department of Biomedical Engineering, Sun Yat-Sen University Shenzhen 518107 China.; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Jiang Y; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Xu S; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Zhang J; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Jung SR; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Yu J; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu., Zhang X; Department of Health Sciences, University of Macau Taipa Macau SAR 999078 China., Chiu DT; Department of Chemistry and Bioengineering, University of Washington Seattle Washington 98195 USA chiu@uw.edu.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 May 22; Vol. 13 (22), pp. 15121-15125. Date of Electronic Publication: 2023 May 22 (Print Publication: 2023).
DOI:	10.1039/d3ra01083j
Abstrakt:	Semiconducting polymer dots (Pdots) with both narrow-band absorption and emission are desirable for multiplexed bioassay applications, but such Pdots with absorption peaks beyond 400 nm are difficult to achieve. Here we describe a donor-energy transfer unit-acceptor (D-ETU-A) design strategy to produce a BODIPY-based Pdot that exhibits simultaneously narrow absorption and emission bands. A green BODIPY (GBDP) unit was employed as the main building block of the polymer backbone, conferring a strong, narrow-band absorption around 551 nm. An NIR720 acceptor provides narrow-band NIR emission. The small Stokes shift of the GBDP donor allows introduction of a benzofurazan-based ETU, resulting in a ternary Pdot with a fluorescence quantum yield of 23.2%, the most efficient yellow-laser excitable Pdot. Due to the strong absorbance band centered at 551 nm and weak absorbance at 405 nm and 488 nm, the Pdot showed high single-particle brightness when excited by a 561 nm (yellow) laser, and selective yellow laser excitation when used to label MCF cells, with much greater brightness when excited at 561 nm than at 405 nm or 488 nm. Competing Interests: The authors declare the following competing financial interests: D. T. Chiu and J. Yu have financial interest in Lamprogen Inc., which has licensed the technology from the University of Washington. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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