Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus.
| Autor: | Yamakawa AC; Biotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil., Haisi A; Biotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil., Kmetiuk LB; Graduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), R dos Funcionários, Curitiba, PR, Brazil., Pellizzaro M; Institute of Collective Health, Federal University of Bahia, Salvador, BA, Brazil., Mendes JCR; Graduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), R dos Funcionários, Curitiba, PR, Brazil., Canavessi AMO; Department of Animal Science, São Paulo University (USP), Piracicaba, SP, Brazil., Ullmann LS; Biotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil., de Castro WAC; Latin-American Institute of Life and Nature Sciences, Federal University for Latin American Integration, Foz do Iguaçu, PR, Brazil., Pessoa Araújo Júnior J; Biotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil., Dos Santos AP; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA., Biondo AW; Graduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), R dos Funcionários, Curitiba, PR, Brazil.; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA. |
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| Jazyk: | angličtina |
| Zdroj: | JFMS open reports [JFMS Open Rep] 2023 Feb 13; Vol. 9 (1), pp. 20551169221148672. Date of Electronic Publication: 2023 Feb 13 (Print Publication: 2023). |
| DOI: | 10.1177/20551169221148672 |
| Abstrakt: | Objectives: The aim of the present study was to assess the frequency of hemoplasma, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections in cats living in an on-campus shelter and free-roaming cats within a university campus in Brazil. Methods: Blood samples were tested using quantitative PCR for hemoplasma, FIV and FeLV. Positive hemoplasma samples were sequenced. Associations between hemoplasma detection and living situation, sex, flea and/or tick parasitism, and coinfection with FIV and FeLV, were assessed using Fisher's exact test and the respective odds ratios were calculated. Results: Overall, 6/45 (13.3%) cats tested positive: four (8.9%) were infected with ' Candidatus Mycoplasma haemominutum' and two (4.4%) with Mycoplasma haemofelis . All positive samples were from free-roaming cats (6/15; 40.0%) and had statistically significantly lower packed cell volumes ( P = 0.037). Although 5/23 (21.7%) males and 1/22 (4.6%) females were positive, no statistically significant association between sex and hemoplasma infection was found ( P = 0.19). Viral quantitative PCR (qPCR) was performed on 43/45 samples, among which 2/43 (4.7%) were positive for FIV and none for FeLV. Only one cat (2.3%) was coinfected with hemoplasma and FIV ( P = 0.26). In addition, 4/6 (66.7%) cats that tested positive for hemoplasmas were infested by fleas ( P = 0.0014) and/or ticks ( P = 0.25). Conclusions and Relevance: These results show that even if the free-roaming cat population is clinically healthy and has adequate access to food, it may present flea infestation and hemoplasma infection with lower packed cell volume values. Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. (© The Author(s) 2023.) |
| Databáze: | MEDLINE |
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