Epstein-Barr virus in gastric cancer and association with 30 bp del-latent membrane protein 1 polymorphism.

Autor:	Santos ERCD; Universidade Federal do Rio de Janeiro, Hospital Universitário Clementino Fraga Filho - Rio de Janeiro (RJ), Brazil., Silva MSDME; Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Departamento de Patologia - Rio de Janeiro (RJ), Brazil., Canedo NHS; Universidade Federal do Rio de Janeiro, Hospital Universitário Clementino Fraga Filho, Medical School, Pathology Department - Rio de Janeiro (RJ), Brazil., Gaui MFD; Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Departamento de Clinica Médica - Rio de Janeiro (RJ), Brazil., Britto ÁLVL; Instituto de Biologia do Exército - Rio de Janeiro (RJ), Brazil., Silva WMVD; Oswaldo Cruz Foundation, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, National Reference Laboratory for Tuberculosis - Rio de Janeiro (RJ), Brazil., Carvalho MDGDC; Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Departamento de Patologia - Rio de Janeiro (RJ), Brazil., Bravo Neto GP; Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Departamento de Cirurgia - Rio de Janeiro (RJ), Brazil.
Jazyk:	angličtina
Zdroj:	Revista da Associacao Medica Brasileira (1992) [Rev Assoc Med Bras (1992)] 2023 May 19; Vol. 69 (5), pp. e20221571. Date of Electronic Publication: 2023 May 19 (Print Publication: 2023).
DOI:	10.1590/1806-9282.20221571
Abstrakt:	Objective: This study aimed to determine the frequencies of Epstein-Barr virus, types 1 and 2 infection, and 30 bp del-latent membrane protein 1 viral polymorphism in gastric adenocarcinomas, as well as to investigate the association between Epstein-Barr virus infection and tumor location, type, and the patient's sex. Methods: Samples were collected from 38 patients treated at a university hospital in Rio de Janeiro, Brazil. Epstein-Barr virus detection and genotyping were performed by polymerase chain reaction, followed by polyacrylamide gel electrophoresis and staining by the silver nitrate method. Results: Overall, 68.4% of patients had Epstein-Barr virus-positive tumors. Of these, 65.4% presented infection by Epstein-Barr virus type 1, 23.1% by Epstein-Barr virus type 2, and 11.5% had coinfection with types 1 and 2. The 30 bp del-latent membrane protein 1 polymorphism was found in 42.3% of Epstein-Barr virus-positive tumors, 23.1% had the wild-type virus, and 23.1% had the wild-type and the polymorphism concomitantly. In 11.5% of Epstein-Barr virus-positive tumors, it was impossible to determine whether there was polymorphism or not. Tumor location in the antrum (22 of 38) and diffuse type (27 of 38) were predominant. There was no significant difference in Epstein-Barr virus infection or the 30 bp del-latent membrane protein 1 polymorphism between men and women. Conclusion: Epstein-Barr virus infection was found in 68.4% of tumors investigated in this study. To the best of our knowledge, this is the first article showing the coinfection of Epstein-Barr virus types 1 and 2 in gastric carcinoma in Brazil.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu