| Autor: |
Borges PP; Postgraduate Program in Biodiversity, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil., Benvenutti L; Postgraduate Program in Pharmaceutical Science, Universidade do Vale do Itajaí, Itajaí, SC, Brazil., Lana Schneider A; Pharmacy Course, Department of Pharmaceutical Sciences, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil., Franke Echterhoff MR; Postgraduate Program in Chemistry, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil., Gonçalves Lopes B; Postgraduate Program in Chemistry, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil., Siebert DA; Postgraduate Program in Chemistry, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Micke GA; Postgraduate Program in Chemistry, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Vitali L; Postgraduate Program in Chemistry, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Alberton MD; Postgraduate Program in Biodiversity, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil.; Postgraduate Program in Chemistry, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil., Quintão NLM; Postgraduate Program in Pharmaceutical Science, Universidade do Vale do Itajaí, Itajaí, SC, Brazil., Santin JR; Postgraduate Program in Pharmaceutical Science, Universidade do Vale do Itajaí, Itajaí, SC, Brazil., Daufenback Machado I; Postgraduate Program in Biodiversity, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil. |
| Abstrakt: |
Myrcia is a genus widespread in South America with many species presenting anti-inflammatory and biological properties. We investigated the anti-inflammatory activity of crude hydroalcoholic extract of Myrcia pubipetala leaves (CHE-MP) using macrophages (RAW 264.7), and the air pouch model in mice to evaluate leukocyte migration and mediator's release. Adhesion molecule expression, CD49 and CD18, was evaluated in neutrophils. In vitro , the CHE-MP significantly reduced nitric oxide (NO), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) levels in the exudate and the supernatant culture. CHE-MP did not present cytotoxicity and modulated the percentage of positive neutrophils for CD18 and its expression per cell, without modifying the expression of CD49, which corroborated with significantly reduced neutrophil migration to inflammatory exudate and subcutaneous tissue. Taken together, the data demonstrate that CHE-MP presents a potential activity on innate inflammatory. |
