SERS Endoscopy for Monitoring Intracellular Drug Dynamics.

Autor: Fortuni B; Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium., Ricci M; Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium., Vitale R; U. Lille, CNRS, LASIRE, Laboratoire Avancé de Spectroscopie pour les Interactions, la Réactivité et l'Environnement, Cité Scientifique, F-59000 Lille, France., Inose T; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan., Zhang Q; Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020, Japan., Hutchison JA; ARC Centre of Excellence in Exciton Science, School of Chemistry, University of Melbourne, Parkville, VIC 3010, Australia., Hirai K; Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020, Japan., Fujita Y; Toray Research Center, Inc., Sonoyama 3-3-7, Otsu, Shiga 520-8567, Japan., Toyouchi S; Research Institute for Light-Induced Acceleration System (RILACS), Osaka Metropolitan University, 1-2 Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan., Krzyzowska S; Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium., Van Zundert I; Synthetic Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands., Rocha S; Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium., Uji-I H; Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium.; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.; Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020, Japan.
Jazyk: angličtina
Zdroj: ACS sensors [ACS Sens] 2023 Jun 23; Vol. 8 (6), pp. 2340-2347. Date of Electronic Publication: 2023 May 23.
DOI: 10.1021/acssensors.3c00394
Abstrakt: Understanding the dynamics and distribution of medicinal drugs in living cells is essential for the design and discovery of treatments. The tools available for revealing this information are, however, extremely limited. Here, we report the application of surface-enhanced Raman scattering (SERS) endoscopy, using plasmonic nanowires as SERS probes, to monitor the intracellular fate and dynamics of a common chemo-drug, doxorubicin, in A549 cancer cells. The unique spatio-temporal resolution of this technique reveals unprecedented information on the mode of action of doxorubicin: its localization in the nucleus, its complexation with medium components, and its intercalation with DNA as a function of time. Notably, we were able to discriminate these factors for the direct administration of doxorubicin or the use of a doxorubicin delivery system. The results reported here show that SERS endoscopy may have an important future role in medicinal chemistry for studying the dynamics and mechanism of action of drugs in cells.
Databáze: MEDLINE