A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats.

Autor: Nicoleti JL; Laboratório de Imunologia E Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State, 40231-300, Brazil., Braga ES; Laboratório de Química Biológica, Instituto de Química, Universidade Estadual de Campinas, Campinas, São Paulo State, 13083-970, Brazil., Stanisic D; Laboratório de Química Biológica, Instituto de Química, Universidade Estadual de Campinas, Campinas, São Paulo State, 13083-970, Brazil., Jadranin M; Laboratório de Química Biológica, Instituto de Química, Universidade Estadual de Campinas, Campinas, São Paulo State, 13083-970, Brazil.; Institute of Chemistry, Technology and Metallurgy, University of Belgrade, 11000, Belgrade, Serbia., Façanha DAE; Institute of Rural Development, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, Ceará State, 62790-000, Brazil., Barral TD; Laboratório de Imunologia E Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State, 40231-300, Brazil., Hanna SA; Laboratório de Imunologia E Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State, 40231-300, Brazil., Azevedo V; Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais State, 31270-901, Brazil., Meyer R; Laboratório de Imunologia E Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State, 40231-300, Brazil., Tasic L; Laboratório de Química Biológica, Instituto de Química, Universidade Estadual de Campinas, Campinas, São Paulo State, 13083-970, Brazil., Portela RW; Laboratório de Imunologia E Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State, 40231-300, Brazil. rwportela@ufba.br.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Jul; Vol. 107 (14), pp. 4593-4603. Date of Electronic Publication: 2023 May 23.
DOI: 10.1007/s00253-023-12595-0
Abstrakt: Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host-pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance ( 1 H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr-Purcell-Meiboom-Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD + , and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis. KEY POINTS: • Sixty-two samples from healthy, CLA asymptomatic, and symptomatic goats were screened • Twenty metabolites of interest were identified by NOESY and 29 by CPMG • 1 H-NMR NOESY and CPMG were complementary and mutually confirming.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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