Ranking mAb-excipient interactions in biologics formulations by NMR spectroscopy and computational approaches.

Autor: Zhang C; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Gossert ST; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Williams J; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Little M; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Barros M; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Dear B; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Falk B; Drug Discovery, Bristol Myers Squibb, Lawrenceville, NJ, USA., Kanthe AD; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Garmise R; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Mueller L; Drug Discovery, Bristol Myers Squibb, Lawrenceville, NJ, USA., Ilott A; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA., Abraham A; Drug Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA.
Jazyk: angličtina
Zdroj: MAbs [MAbs] 2023 Jan-Dec; Vol. 15 (1), pp. 2212416.
DOI: 10.1080/19420862.2023.2212416
Abstrakt: Excipients are added to biopharmaceutical formulations to enhance protein stability and enable the development of robust formulations with acceptable physicochemical properties, but the mechanism by which they confer stability is not fully understood. Here, we aimed to elucidate the mechanism through direct experimental evidence of the binding affinity of an excipient to a monoclonal antibody (mAb), using saturation transfer difference (STD) nuclear magnetic resonance (NMR) spectroscopic method. We ranked a series of excipients with respect to their dissociation constant (K D ) and nonspecific binding constants (N s ). In parallel, molecular dynamic and site identification by ligand competitive saturation (SILCS)-Monte Carlo simulations were done to rank the excipient proximity to the proteins, thereby corroborating the ranking by STD NMR. Finally, the excipient ranking by NMR was correlated with mAb conformational and colloidal stability. Our approach can aid excipient selection in biologic formulations by providing insights into mAb-excipient affinities before conventional and time-consuming excipient screening studies are conducted.
Databáze: MEDLINE
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