Genome-centric polyhydroxyalkanoate reconciliation reveals nutrient enriched growth dependent biosynthesis in Bacillus cereus IBA1.

Autor: Kumar R; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong., Li D; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong., Luo L; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong., Manu MK; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong., Zhao J; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong., Tyagi RD; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong., Wong JWC; Institute of Bioresource and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong; Research Centre for Eco-environmental Engineering, Dongguan University of Technology, Dongguan 523830, PR China. Electronic address: jwcwong@hkbu.edu.hk.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2023 Aug; Vol. 382, pp. 129210. Date of Electronic Publication: 2023 May 20.
DOI: 10.1016/j.biortech.2023.129210
Abstrakt: Microbiological polyhydroxyalkanoates (PHAs) are rooted as the most promising bio-replacements of synthetic polymers. Inherent properties of these PHAs further expand their applicability in numerous industrial, environmental, and clinical sectors. To propel these, a new environmental, endotoxin free gram-positive bacterium i.e., Bacillus cereus IBA1 was identified to harbor advantageous PHA producer characteristics through high-throughput omics mining approaches. Unlike traditional fermentations, nutrient enriched strategy was used to enhance PHA granular concentrations by ∼2.3 folds to 2.78 ± 0.19 g/L. Additionally, this study is the first to confirm an underlying growth dependent PHA biogenesis through exploring PHA granule associated operons which harbour constitutively expressing PHA synthase (phaC) coupled with differentially expressing PHA synthase subunit (phaR) and regulatory protein (phaP, phaQ) amid different growth phases. Moreover, the feasibility of this promising microbial phenomenon could propel next-generation biopolymers, and increase industrial applicability of PHAs, thereby significantly contributing to the sustainable development.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: This research work contributed towards a provisional patent pending application number 202211133387.9 under China National Intellectual Property Administration. However, it has not affected the scope or outcomes of the present research.
(Copyright © 2023. Published by Elsevier Ltd.)
Databáze: MEDLINE
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