Comparison between transrectal palpation, B-mode and Doppler ultrasonography to assess luteal function in Holstein cattle.

Autor:	Yáñez U; Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, Lugo, Spain., Murillo AV; Friends of Animals Veterinary Hospital Srl, Latina, Italy., Becerra JJ; Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, Lugo, Spain., Herradón PG; Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, Lugo, Spain., Peña AI; Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, Lugo, Spain., Quintela LA; Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, Lugo, Spain.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 May 04; Vol. 10, pp. 1162589. Date of Electronic Publication: 2023 May 04 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1162589
Abstrakt:	Introduction: Over the years, the most common methods for monitoring reproductive health in cattle have varied from transrectal palpation to B-mode ultrasonography. Nowadays, some portable ultrasound equipment includes the Doppler mode. Therefore, the aim of this study was to compare the accuracy of the different methods to assess corpus luteum (CL) functionality. Methods: In Experiment 1, 53 Holstein lactating cows undergoing a synchronization protocol were examined via transrectal palpation and B-mode scanning. Measurements for the largest diameter (LAD) and subjective size of CL (SCLS) were collected. Data were analyzed using correlation analysis and ROC Curves. In Experiment 2, 30 Holstein non-lactating cows with a CL were administered PGF2α and examined several times after injection, first in B-mode and then with Power Doppler. Measurements for LAD, CL area (CLA) and subjective and objective CL blood flow were collected. Blood samples were taken in both experiments to determine P4 concentration. Data were analyzed using correlation analysis and the GLM repeated measures test. Results: Results for Experiment 1 showed that LAD was more accurate than SCLS. In Experiment 2, CLA was the best measurement to assess CL function, although both subjective and objective CL blood flow offer accurate information 24 h after PGF2α administration. Discussion: Consequently, ultrasonography provides more accurate information about CL function than transrectal palpation. Although CLA seems to be an earlier indicator of luteal function than blood flow, 24 h after the onset of luteolysis, both parameters are valid. Competing Interests: Author AM is employed by Friends of Animals Veterinary Hospital Srl. (Italy). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Yáñez, Murillo, Becerra, Herradón, Peña and Quintela.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu