| Autor: |
Taylor MA; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Kandyba E; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Halliwill K; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Delrosario R; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Koroshkin M; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Goodarzi H; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Quigley D; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Li YR; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Wu D; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Bollam S; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Mirzoeva O; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Akhurst RJ; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA., Balmain A; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco; San Francisco, 94158, USA. |
| Abstrakt: |
Adult mammalian stem cells play critical roles in normal tissue homeostasis, as well as in tumor development, by contributing to cell heterogeneity, plasticity, and development of drug resistance. The relationship between different types of normal and cancer stem cells is highly controversial and poorly understood. Here, we carried out gene expression network analysis of normal and tumor samples from genetically heterogeneous mice to create network metagenes for visualization of stem-cell networks, rather than individual stem-cell markers, at the single-cell level during multistage carcinogenesis. We combined this approach with lineage tracing and single-cell RNASeq of stem cells and their progeny, identifying a previously unrecognized hierarchy in which Lgr6 + stem cells from tumors generate progeny that express a range of other stem-cell markers including Sox2, Pitx1, Foxa1, Klf5 , and Cd44. Our data identify a convergence of multiple stem-cell and tumor-suppressor pathways in benign tumor cells expressing markers of lineage plasticity and oxidative stress. This same single-cell population expresses network metagenes corresponding to markers of cancer drug resistance in human tumors of the skin, lung and prostate. Treatment of mouse squamous carcinomas in vivo with the chemotherapeutic cis -platin resulted in elevated expression of the genes that mark this cell population. Our data have allowed us to create a simplified model of multistage carcinogenesis that identifies distinct stem-cell states at different stages of tumor progression, thereby identifying networks involved in lineage plasticity, drug resistance, and immune surveillance, providing a rich source of potential targets for cancer therapy. |
