Performance evaluation of mesoscopic photoacoustic imaging.

Autor:	Hacker L; Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK.; Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge CB2 0RE, UK., Brown EL; Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK.; Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge CB2 0RE, UK., Lefebvre TL; Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK.; Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge CB2 0RE, UK., Sweeney PW; Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK.; Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge CB2 0RE, UK., Bohndiek SE; Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK.; Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge CB2 0RE, UK.
Jazyk:	angličtina
Zdroj:	Photoacoustics [Photoacoustics] 2023 May 06; Vol. 31, pp. 100505. Date of Electronic Publication: 2023 May 06 (Print Publication: 2023).
DOI:	10.1016/j.pacs.2023.100505
Abstrakt:	Photoacoustic mesoscopy visualises vascular architecture at high-resolution up to ~3 mm depth. Despite promise in preclinical and clinical imaging studies, with applications in oncology and dermatology, the accuracy and precision of photoacoustic mesoscopy is not well established. Here, we evaluate a commercial photoacoustic mesoscopy system for imaging vascular structures. Typical artefact types are first highlighted and limitations due to non-isotropic illumination and detection are evaluated with respect to rotation, angularity, and depth of the target. Then, using tailored phantoms and mouse models, we investigate system precision, showing coefficients of variation (COV) between repeated scans [short term (1 h): COV= 1.2%; long term (25 days): COV= 9.6%], from target repositioning (without: COV=1.2%, with: COV=4.1%), or from varying in vivo user experience (experienced: COV=15.9%, unexperienced: COV=20.2%). Our findings show robustness of the technique, but also underscore general challenges of limited-view photoacoustic systems in accurately imaging vessel-like structures, thereby guiding users when interpreting biologically-relevant information. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests Sarah Bohndiek reports a relationship with EPFL Center for Biomedical Imaging that includes: speaking and lecture fees. Sarah Bohndiek reports a relationship with PreXion Inc that includes: funding grants. Sarah Bohndiek reports a relationship with iThera Medical GmbH that includes: non-financial support.The authors declare the following financial interests / personal relationships, which may be considered as potential competing interests. Sarah Bohndiek reports a relationship with EPFL Center for Biomedical Imaging that includes: speaking and lecture fees. Sarah Bohndiek reports a relationship with PreXion Inc that includes: funding grants. Sarah Bohndiek reports a relationship with iThera Medical GmbH that includes: non-financial support. The other authors have no conflict of interest related to the present manuscript to disclose. (© 2023 The Authors. Published by Elsevier GmbH.)
Databáze:	MEDLINE
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