Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues.
| Autor: | Rosti V; Institute of Biomedical Technologies, National Research Council, Milan, Italy.; Istituto Nazionale di Genetica Molecolare 'Romeo ed Enrica Invernizzi', INGM, Milan, Italy., Gorini F; Istituto Nazionale di Genetica Molecolare 'Romeo ed Enrica Invernizzi', INGM, Milan, Italy., Santarelli P; Istituto Nazionale di Genetica Molecolare 'Romeo ed Enrica Invernizzi', INGM, Milan, Italy., Sarnicola ML; Istituto Nazionale di Genetica Molecolare 'Romeo ed Enrica Invernizzi', INGM, Milan, Italy., Magnani S; Charles River Laboratories Italia S.r.l., Milan, Italy., Lanzuolo C; Institute of Biomedical Technologies, National Research Council, Milan, Italy. chiara.lanzuolo@cnr.it.; Istituto Nazionale di Genetica Molecolare 'Romeo ed Enrica Invernizzi', INGM, Milan, Italy. chiara.lanzuolo@cnr.it. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2655, pp. 125-146. |
| DOI: | 10.1007/978-1-0716-3143-0_11 |
| Abstrakt: | The regulation of chromatin structure depends on a dynamic, multiple mechanisms that modulate gene expression and constitute the epigenome. The Polycomb group (PcG) of proteins are epigenetic factors involved in the transcriptional repression. Among their multilevel, chromatin-associated functions, PcG proteins mediate the establishment and maintenance of higher-order structures at target genes, allowing the transmission of transcriptional programs throughout the cell cycle.In the nucleus, PcG proteins localize close to the pericentric heterochromatin forming microscopically foci, called Polycomb bodies. Here, to visualize the tissue-specific PcG distribution in the aorta, dorsal skin and hindlimb muscles, we combine a fluorescence-activated cell sorter (FACS)-based method with an immunofluorescence staining. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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