Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice.

Autor: Noonan AM; Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada., Buliung E; Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada., Briar KJ; Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada., Quinonez D; Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Bone and Joint Institute, University of Western Ontario, London, ON, Canada., Séguin CA; Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Bone and Joint Institute, University of Western Ontario, London, ON, Canada., Brown SHM; Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada. shmbrown@uoguelph.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 20; Vol. 13 (1), pp. 8170. Date of Electronic Publication: 2023 May 20.
DOI: 10.1038/s41598-023-35506-9
Abstrakt: Degenerative spinal disorders, including kyphotic deformity, are associated with a range of degenerative characteristics of the paraspinal musculature. It has therefore been hypothesized that paraspinal muscular dysfunction is a causative factor for degenerative spinal deformity; however, experimental studies demonstrating causative relationships are lacking. Male and female mice received either glycerol or saline injections bilaterally along the length of the paraspinal muscles at four timepoints, each separated by 2 weeks. Immediately after sacrifice, micro-CT was performed to measure spinal deformity; paraspinal muscle biopsies were taken to measure active, passive and structural properties; and lumbar spines were fixed for analysis of intervertebral disc (IVD) degeneration. Glycerol-injected mice demonstrated clear signs of paraspinal muscle degeneration and dysfunction: significantly (p < 0.01) greater collagen content, lower density, lower absolute active force, greater passive stiffness compared to saline-injected mice. Further, glycerol-injected mice exhibited spinal deformity: significantly (p < 0.01) greater kyphotic angle than saline-injected mice. Glycerol-injected mice also demonstrated a significantly (p < 0.01) greater IVD degenerative score (although mild) at the upper-most lumbar level compared to saline-injected mice. These findings provide direct evidence that combined morphological (fibrosis) and functional (actively weaker and passively stiffer) alterations to the paraspinal muscles can lead to negative changes and deformity within the thoracolumbar spine.
(© 2023. The Author(s).)
Databáze: MEDLINE
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