Quercetin abrogates lipopolysaccharide-induced depressive-like symptoms by inhibiting neuroinflammation via microglial NLRP3/NFκB/iNOS signaling pathway.

Autor: Adeoluwa OA; Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria. Electronic address: adeoluwaolusegun@yahoo.com., Olayinka JN; Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Adeoluwa GO; Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Akinluyi ET; Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Adeniyi FR; Department of Pharmacology and Toxicology, College of Pharmacy, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Fafure A; Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Nebo K; Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Edem EE; Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria., Eduviere AT; Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Delta State University Abraka, Delta State, Nigeria., Abubakar B; Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medicine and Health Sciences, Afe Babalola University Ado Ekiti, Ekiti State, Nigeria.
Jazyk: angličtina
Zdroj: Behavioural brain research [Behav Brain Res] 2023 Jul 26; Vol. 450, pp. 114503. Date of Electronic Publication: 2023 May 18.
DOI: 10.1016/j.bbr.2023.114503
Abstrakt: Background: Major depressive disorder is a serious psychiatric illness having serious damaging effect on the quality of life of suffers. Quercetin is a plant flavonoid, mostly used as a constituent in dietary products. This study evaluated antidepressant effect of quercetin on lipopolysaccharide (LPS)-induced depression in rats.
Materials and Methods: Twenty-one male rats were randomly assigned into three groups (n = 7): group 1 (vehicle only), group 2 (quercetin), group 3 (LPS). Rats were treated with vehicle (10 mL/kg, p.o.) or quercetin (50 mg/kg, p.o.) for seven days. Sixty minutes after treatment on day seven, all animals were injected with LPS (0.83 mg/kg, i.p.) except group 1 (vehicle only). Twenty-four hours after LPS injection, animals were assessed for depressive symptoms using forced swim, sucrose splash and open field tests. Animals were sacrificed; brain samples collected for bioassay of pro-inflammatory mediators, TNF-α, IL-6 and IL-17 were measured using enzyme-linked immunosorbent assay (ELISA) while expressions of NF-κB, inflammasomes, microglia and iNOS were quantified by immunohistochemistry.
Results: The LPS significantly (p < 0.05) decreased mobility of rats in FST and decreased sucrose preference, which is indicative of depressive-like behaviours. These behaviours were significantly (p < 0.05) attenuated by quercetin compared to control (vehicle only). Following LPS exposure, the expressions of inflammasomes, NF-κB, iNOS, proinflammatory cytokines and microglia positive cells in the hippocampus and prefrontal cortex were significantly (p < 0.05) elevated. All these were attenuated by pretreating animals with quercetin.
Conclusion: Quercetin exhibit antidepressant-like property, which may be related to inhibition of neuroinflammatory signaling pathways.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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