Development of a real-time RT-PCR system applicable for rapid and pen-side diagnosis of foot-and-mouth disease using a portable device, PicoGene® PCR1100.

Autor: Matsui Y; Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan., Chottikamporn J; Department of Livestock Development, Regional Reference Laboratory for Foot and Mouth Disease in the South East Asia, Pakchong, 30130, Thailand., Ungvanijban S; Department of Livestock Development, Regional Reference Laboratory for Foot and Mouth Disease in the South East Asia, Pakchong, 30130, Thailand., Seeyo KB; Department of Livestock Development, Regional Reference Laboratory for Foot and Mouth Disease in the South East Asia, Pakchong, 30130, Thailand., Vitoonpong R; Virology Section, Department of Livestock Development, National Institute of Animal Health, Bangkok 10400, Thailand., Suwankitwat N; Virology Section, Department of Livestock Development, National Institute of Animal Health, Bangkok 10400, Thailand., Songkasupa T; Virology Section, Department of Livestock Development, National Institute of Animal Health, Bangkok 10400, Thailand., Norimine J; Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan; Laboratory of Animal Infectious Disease and Prevention Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan., Yamada K; Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan; Laboratory of Veterinary Public Health, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan., Chintapitaksakul L; Bureau of Quality Control of Livestock Products, Bang Kadi, Mueang Pathum Thani District, Pathum Thani 12000, Thailand., Misawa N; Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan; Laboratory of Veterinary Public Health, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki City, Miyazaki 889-2192, Japan. Electronic address: a0d901u@cc.miyazaki-u.ac.jp.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2023 Sep; Vol. 319, pp. 114753. Date of Electronic Publication: 2023 May 18.
DOI: 10.1016/j.jviromet.2023.114753
Abstrakt: Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease, causing devastating losses to the livestock industry. A diagnostic method that enables quick decisions is required to control the disease, especially in FMD-free countries. Although conventional real-time reverse transcription polymerase chain reaction (RT-PCR) is a highly sensitive method widely used for the diagnosis of FMD, a time lag caused by the transport of samples to a laboratory may allow the spread of FMD. Here, we evaluated a real-time RT-PCR system using a portable PicoGene PCR1100 device for FMD diagnosis. This system could detect the synthetic FMD viral RNA within 20 min with high sensitivity compared to a conventional real-time RT-PCR. Furthermore, the Lysis Buffer S for crude nucleic extraction improved the viral RNA detection of this system in a homogenate of vesicular epithelium samples collected from FMD virus-infected animals. Furthermore, this system could detect the viral RNA in crude extracts prepared using the Lysis Buffer S from the vesicular epithelium samples homogenized using a Finger Masher tube, which allows easy homogenization without any equipment, with a high correlation compared to the standard method. Thus, the PicoGene device system can be utilized for the rapid and pen-side diagnosis of FMD.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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