Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source.

Autor: Gul M; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Ayan E; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Destan E; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Johnson JA; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Shafiei A; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Kepceoğlu A; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Koç University Surface Science and Technology Center (KUYTAM), Koç University, Istanbul, Türkiye., Yilmaz M; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Ertem FB; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Yapici İ; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Tosun B; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Baldir N; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Tokay N; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Nergiz Z; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Koç University Isbank Center for Infectious Diseases (KUISCID), Koç University, Istanbul, Türkiye., Karakadioğlu G; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Paydos SS; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Kulakman C; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Ferah CK; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Güven Ö; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Atalay N; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, Kocaeli, Türkiye.; Experimental Medicine Application & Research Center, University of Health Sciences Türkiye, Istanbul, Türkiye., Akcan EK; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Technical University, Istanbul, Türkiye., Cetinok H; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Arslan NE; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Arel University, Istanbul, Türkiye., Şabanoğlu K; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Yıldız Technical University, Istanbul, Türkiye., Aşci B; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Tavli S; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Gümüsboğa H; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye., Altuntaş S; Experimental Medicine Application & Research Center, University of Health Sciences Türkiye, Istanbul, Türkiye.; Department of Tissue Engineering, Hamidiye Institute of Health Sciences, University of Health Sciences Türkiye, Istanbul, Türkiye., Otsuka M; Medicinal and Biological Chemistry Science Farm Joint Research Laboratory, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.; Department of Drug Discovery, Science Farm Ltd., Kumamoto, Japan., Fujita M; Medicinal and Biological Chemistry Science Farm Joint Research Laboratory, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan., Teki N Ş; Experimental Medicine Application & Research Center, University of Health Sciences Türkiye, Istanbul, Türkiye.; The Scientific and Technological Research Council of Türkiye (TÜBİTAK) Marmara Research Center (MAM), Life Sciences, Kocaeli, Türkiye.; Department of Basic Medical Sciences, Division of Medical Biology, Faculty of Medicine, University of Health Sciences Türkiye, Istanbul, Türkiye., Çi Ftçi H; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.; Medicinal and Biological Chemistry Science Farm Joint Research Laboratory, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.; Department of Drug Discovery, Science Farm Ltd., Kumamoto, Japan., Durdaği S; Department of Biophysics, School of Medicine, Bahcesehir University, Istanbul, Türkiye., Karaca E; Izmir Biomedicine and Genome Center, Izmir, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Türkiye., Kaplan Türköz B; Department of Food Engineering, Faculty of Engineering, Ege University, Izmir, Türkiye., Kabasakal BV; Turkish Accelerator and Radiation Laboratory (TARLA), Ankara University, Ankara, Türkiye.; School of Biochemistry, University of Bristol, Bristol, UK., Kati A; Experimental Medicine Application & Research Center, University of Health Sciences Türkiye, Istanbul, Türkiye.; Department of Biotechnology, Hamidiye Institute of Health Sciences, University of Health Sciences Türkiye, Istanbul, Türkiye., DeMi Rci H; Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye. hdemirci@ku.edu.tr.; Koç University Isbank Center for Infectious Diseases (KUISCID), Koç University, Istanbul, Türkiye. hdemirci@ku.edu.tr.; SLAC National Laboratory, Stanford PULSE Institute, Menlo Park, CA, USA. hdemirci@ku.edu.tr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 19; Vol. 13 (1), pp. 8123. Date of Electronic Publication: 2023 May 19.
DOI: 10.1038/s41598-023-33989-0
Abstrakt: High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 Å and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.
(© 2023. The Author(s).)
Databáze: MEDLINE
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