Trichinocidal activity of a novel formulation of curcumin-olive oil nanocomposite in vitro.

Autor: Khalifa MM; Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt., Ramadan RM; Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt. Electronic address: reem.montaser@cu.edu.eg., Youssef FS; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Egypt., Auda HM; Department of Medicine & Infectious Diseases, Faculty of Vet. Medicine, Cairo University, Egypt., El-Bahy MM; Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt., Taha NM; Department of Parasitology, Faculty of Medicine, Cairo University, Egypt.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2023 Jun; Vol. 41, pp. 100880. Date of Electronic Publication: 2023 Apr 15.
DOI: 10.1016/j.vprsr.2023.100880
Abstrakt: Curcumin-olive oil nanocomposite (CO-NC), a novel formulation of nano-curcumin, was produced and characterized. By evaluating the death rate and DNA damage inflicted on adult Trichinella spiralis (T. spiralis) worms using the comet test and Scanning electron microscopy (SEM) analysis, the effectiveness of the substance against these worms was assessed in vitro. The mortality effects of CO-NC on the parasite adult worms were increased with the upgrading in the concentration and exposure time from 1 to 24 h using concentrations from 10 to 100 ppm. LC 50 was determined to be 10.0 ppm/18 h, 20.0 ppm/9 h, 40.0 ppm/6 h, 80.0 ppm/2 h, and 100.0 ppm/1 h, while LC 100 was 40.0 ppm/24 h, 80.0 ppm/12 h, and 100.0 ppm/6 h. The comet assay was utilized to examine DNA damage in control and dead worms exposed to varying doses. A direct correlation (P ≤ 0.05) was found between the increase in CO-NC dose and the degree of DNA damage as indicated by alterations in DNA % in the tail segment, tail length (μm), tail moment (μm), and olive tail moment with the control samples. The sub-epidermal layer was detached, the cuticle was partially sloughed off, and the usual creases, ridges, and annulations were altered in the T. spiralis exposed worms. As a result, the tested new trichinocidal drug formulation of nano-curcumin on an oil base was confirmed to be an efficient, secure, and environmentally friendly alternative. The medication has the potential to severely and irreversibly harm the DNA and ultrastructural morphology of adult worms.
Competing Interests: Declaration of Competing Interest There are no declared conflicts of interest. The corresponding author is ready to provide any detailed data upon request.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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