Melatonin promotes orofacial antinociception in adult zebrafish by modulating TRP channels.

Autor: Sampaio GMMS; Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil., Vieira-Neto AE; Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil., Leite GO; Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil., Campos AR; Experimental Biology Center, University of Fortaleza, Fortaleza, Brazil. Electronic address: adrirolim@unifor.br.
Jazyk: angličtina
Zdroj: Physiology & behavior [Physiol Behav] 2023 Oct 01; Vol. 269, pp. 114238. Date of Electronic Publication: 2023 May 18.
DOI: 10.1016/j.physbeh.2023.114238
Abstrakt: Melatonin is an endogenous hormone, known as the sleep hormone, which has already demonstrated its antinociceptive effect. This study aimed to evaluate the participation of TRP's channels in the orofacial antinociceptive effect of melatonin (MT) in adult zebrafish. Initially, the open field test was performed to evaluate the effect of MT on the locomotor activity of adult zebrafish. Then, the animals were pre-treated with MT (0.1, 0.3 or 1 mg/mL; gavage) and acute orofacial nociception was induced by the application of capsaicin (TRPV1 agonist), cinnamaldehyde (TRPA1 agonist) or menthol (TRPM8 agonist) applied into the animal's lip. Naive groups were included. MT, per se, did not alter the locomotor activity of the animals. MT reduced the nociceptive behavior induced by the three agonists; however, the most significant effect was obtained with the lowest concentration tested (0.1 mg/mL) in the capsaicin test. The orofacial antinociceptive effect of melatonin was prevented by capsazepine (TRPV1 antagonist), but not by HC-030031 (TRPA1 antagonist). The molecular docking study indicated interaction between MT and the TRPV1, TRPA1 and TRPM8 channels and, in line with the in vivo results, there was greater affinity between MT and the TRPV1 channel. The results confirm the pharmacological relevance of melatonin as an inhibitor of orofacial nociception and this effect seems to be related to the modulation of TRP's channels.
Competing Interests: Declaration of Competing Interest The authors declare that there is no conflict of interest.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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