Modulating the interaction of graphenic substrates with human interleukin-6 and its monoclonal antibody: a study by Raman images.

Autor:	de la O-Cuevas E; Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas 98068 Zacatecas Mexico.; Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México Circuito Exterior S/N, Cd. Universitaria 04510 Ciudad de México Mexico jose.saniger@icat.unam.mx.; Unidad Académica de Física, Universidad Autónoma de Zacatecas 98068 Zacatecas Mexico., Islas SR; Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México Circuito Exterior S/N, Cd. Universitaria 04510 Ciudad de México Mexico jose.saniger@icat.unam.mx., Gallegos-Flores P; Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas 98068 Zacatecas Mexico., E L EI; Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas 98068 Zacatecas Mexico., Tototzintle-Huitle H; Unidad Académica de Física, Universidad Autónoma de Zacatecas 98068 Zacatecas Mexico., Saniger JM; Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México Circuito Exterior S/N, Cd. Universitaria 04510 Ciudad de México Mexico jose.saniger@icat.unam.mx.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 May 17; Vol. 13 (22), pp. 15114-15120. Date of Electronic Publication: 2023 May 17 (Print Publication: 2023).
DOI:	10.1039/d3ra01627g
Abstrakt:	Interleukin-6 (IL-6) is a cytokine with wide-ranging biological effects, playing an important role on the immune system and inflammatory responses. Therefore, it is important to develop alternative, highly sensitive and reliable analytical methodologies for the accurate detection of this biomarker in biological fluids. Graphene substrates (GS), such as pristine graphene (G), graphene oxide (GO), and reduced graphene oxide (rGO), have shown great benefits for biosensing and in the development of novel biosensor devices. In this work, we present a proof of concept for the development of a new analytical platform for the specific recognition of human interleukin-6, that is based on the coffee-ring formation of monoclonal antibodies of interleukin-6 (mabIL-6) onto amine functionalized GS. The prepared GS/mabIL-6/IL-6 systems were successfully used to show that IL-6 was specifically and selectively adsorbed onto the area of the mabIL-6 coffee-ring. Raman imaging was confirmed as a versatile tool to investigate different antigen-antibody interactions and their surface distribution. This experimental approach can be used to develop a wide variety of substrates for antigen-antibody interaction allowing the specific detection of an analyte in a complex matrix. Competing Interests: There are no conflicts of interest to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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