Exposure to Mancozeb results in increased MAPK phosphorylation and locomotor deficits in zebrafish larvae.

Autor: Martins IK; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil., Pereira LG; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil., Nunes MEM; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil; Postgraduate Program in Biological Sciences - Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil., Lopes AR; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil., Leandro LP; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil; Postgraduate Program in Biological Sciences - Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil., de Mello RS; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil., Mariano MVT; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil., Franco JL; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil., Posser T; Oxidative Stress and Cell Signaling Research Group, Interdisciplinary Center for Biotechnology Research - CIPBIOTEC, Campus São Gabriel, Federal University of Pampa, São Gabriel, RS 97300-000, Brazil. Electronic address: thaisposser@unipampa.edu.br.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2023 Aug; Vol. 270, pp. 109659. Date of Electronic Publication: 2023 May 17.
DOI: 10.1016/j.cbpc.2023.109659
Abstrakt: Mancozeb is a widely used fungicide whose toxicity has been reported in non-target organisms, being considered to have high or very high acute toxicity to aquatic organisms. However, the toxicity of this compound is not well characterized in the developmental stages of fish. In this study, Danio rerio with 4-, 5-, and 6-days post fertilization (dpf) was exposed to MZ at non-lethal concentrations for 24, 48, or 72 h and subsequently, behavioral alterations, oxidative stress parameters and ERK, p38 MAPK , and Akt phosphorylation were analyzed. MZ exposure during the larval period decreased motor performance evaluated by traveled distance, immobile time, and time spent in the peripheral area. In parallel, MZ induced ROS levels and increased the number of cells in apoptosis, causing severe DNA damage, inducing Acetylcholinesterase and Superoxide dismutase activities, and inhibiting Glutathione peroxidase and thioredoxin reductase. Additionally, phosphorylation levels of the proteins p38 MAPK , ERK2, and Akt were stimulated. These findings are relevant considering the ecological implications of MZ exposure to fishes in different developmental stages and the role of the MAPK pathway in events like development and cell death.
Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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