Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy.
| Autor: | Kaur H; Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France., Teulon JM; Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France., Foucher AE; Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France., Fenel D; Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France., Chen SW; Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France; Rue Cyprien Jullin, 38470 Vinay, France., Godon C; Aix Marseille Université, CNRS, CEA, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA Cadarache, 13115 Saint-Paul lez-Durance, France., Desnos T; Aix Marseille Université, CNRS, CEA, Institut de Biosciences et Biotechnologies Aix-Marseille, Equipe Bioénergies et Microalgues, CEA Cadarache, 13115 Saint-Paul-lez-Durance, France. Electronic address: thierry.desnos@cea.fr., Pellequer JL; Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France. Electronic address: jean-luc.pellequer@ibs.fr. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 May 16; Vol. 4 (2), pp. 102265. Date of Electronic Publication: 2023 May 16. |
| DOI: | 10.1016/j.xpro.2023.102265 |
| Abstrakt: | Stiffness plays a central action in plant cell extension. Here, we present a protocol to detect changes in stiffness on the external epidermal cell wall of living plant roots using atomic force microscopy (AFM). We provide generalized instructions for collecting force-distance curves and analysis of stiffness using contact-based mechanical model. With this protocol, and some initial training in AFM, a user is able to perform indentation experiments on 4- and 5-day-old Arabidopsis thaliana and determine stiffness properties. For complete details on the use and execution of this protocol, please refer to Godon et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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