| Autor: |
Vyavahare S; Department of Cell biology and Anatomy, Augusta University, Augusta, GA, USA., Kumar S; Department of Cell biology and Anatomy, Augusta University, Augusta, GA, USA., Smith K; Department of Physiology & Cell Biology, University of Arkansas for Medical Sciences, Arkansas, USA., Mendhe B; Department of Cell biology and Anatomy, Augusta University, Augusta, GA, USA., Zhong R; Department of Neuroscience and Regenerative Medicine, Augusta, GA, USA., Cooley MA; Department of Oral Biology and Diagnostic Sciences, Augusta University, Augusta, GA, USA., Baban B; Department of Oral Biology and Diagnostic Sciences, Augusta University, Augusta, GA, USA., Isales CM; Department of Medicine, Augusta University, Augusta, GA, USA.; Center for Healthy Aging, Augusta University, Augusta, GA, USA.; Department of Neuroscience and Regenerative Medicine, Augusta, GA, USA., Hamrick M; Department of Cell biology and Anatomy, Augusta University, Augusta, GA, USA.; Center for Healthy Aging, Augusta University, Augusta, GA, USA., Hill WD; Department of Pathology and Laboratory Medicine, Medical University of South Carolina, SC 29403, USA., Fulzele S; Department of Cell biology and Anatomy, Augusta University, Augusta, GA, USA.; Department of Medicine, Augusta University, Augusta, GA, USA.; Center for Healthy Aging, Augusta University, Augusta, GA, USA.; Department of Neuroscience and Regenerative Medicine, Augusta, GA, USA. |
| Abstrakt: |
Emerging evidence shows that the microRNA-141-3p is involved in various age-related pathologies. Previously, our group and others reported elevated levels of miR-141-3p in several tissues and organs with age. Here, we inhibited the expression of miR-141-3p using antagomir (Anti-miR-141-3p) in aged mice and explored its role in healthy aging. We analyzed serum (cytokine profiling), spleen (immune profiling), and overall musculoskeletal phenotype. We found decreased levels of pro-inflammatory cytokines (such as TNF-α, IL-1β, IFN-γ) in serum with Anti-miR-141-3p treatment. The flow-cytometry analysis on splenocytes revealed decreased M1 (pro-inflammatory) and increased M2 (anti-inflammatory) populations. We also found improved bone microstructure and muscle fiber size with Anti-miR-141-3p treatment. Molecular analysis revealed that miR-141-3p regulates the expression of AU-rich RNA-binding factor 1 (AUF1) and promotes senescence (p21, p16) and pro-inflammatory (TNF-α, IL-1β, IFN-γ) environment whereas inhibiting miR-141-3p prevents these effects. Furthermore, we demonstrated that the expression of FOXO-1 transcription factor was reduced with Anti-miR-141-3p and elevated with silencing of AUF1 (siRNA-AUF1), suggesting crosstalk between miR-141-3p and FOXO-1. Overall, our proof-of-concept study demonstrates that inhibiting miR-141-3p could be a potential strategy to improve immune, bone, and muscle health with age. |
