Effects of different subcutaneous sites on heterotopic autotransplantation of canine ovarian tissue.

Autor: Brandão FA; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Av. Dr. Silas Munguba, Campus Itaperi. Fortaleza-CE- 60, Fortaleza, CE, 1700, 714-903, Brazil., de Brito DC; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Pereira LM; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Av. Dr. Silas Munguba, Campus Itaperi. Fortaleza-CE- 60, Fortaleza, CE, 1700, 714-903, Brazil., Alves KA; Ovid Research Company, Berkeley, CA, USA., Ñaupas LV; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., de Souza SS; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Av. Dr. Silas Munguba, Campus Itaperi. Fortaleza-CE- 60, Fortaleza, CE, 1700, 714-903, Brazil., de S Cunha DM; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Av. Dr. Silas Munguba, Campus Itaperi. Fortaleza-CE- 60, Fortaleza, CE, 1700, 714-903, Brazil., de S Filho RP; Cardoso Veterinary Hospital, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Alves BG; Ovid Research Company, Berkeley, CA, USA., Rodrigues AP; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Teixeira DI; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Av. Dr. Silas Munguba, Campus Itaperi. Fortaleza-CE- 60, Fortaleza, CE, 1700, 714-903, Brazil. darcio.teixeira@uece.br.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2023 Dec; Vol. 47 (4), pp. 1893-1905. Date of Electronic Publication: 2023 May 18.
DOI: 10.1007/s11259-023-10139-5
Abstrakt: Ovarian tissue transplantation makes it possible to restore fertility; however, the success of this technique depends on the transplant region used. Therefore, this study aimed to evaluate the effect of two subcutaneous regions on canine ovarian transplantation, pinna (Pi) and neck (Ne), for 7 and 15 days. Ovaries collected by ovariosalpingohysterectomy were fragmented using a punch device. Fresh fragments were fixed, and the others were immediately grafted onto the animal itself in the Pi and Ne regions for 7 and 15 days. Recovered fragments were evaluated for histology (morphology, development and stromal density), picrosirius (collagen fibers), and immunohistochemistry (fibrosis and cell proliferation). The results showed that follicular normality rates were lower in Pi-7 (78%) vs. control (90%) and Pi-15 (86%), similar in Ne-7 (92%) and superior in Ne-15 (97%) compared to the control, with the effect of the region Ne (94%) superior (P < 0.05) to Pi (82%). Stromal density reduced in both regions vs. control but was similar within 15 days. Fragments from both regions showed higher fibronectin labeling and deposition of type I and lower type III collagen fibers (P < 0.05) vs. control. Proliferation rates in Ne-7 were higher (P < 0.05) than in control, and Pi-15 was higher (P < 0.05) than Ne-15. In conclusion, the pinna may be a region with greater potential than the neck after a 15-day autotransplantation of canine ovarian tissue.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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