Impact of vitamin D in children with chronic tonsillitis (immunohistochemical study of CD68 polarisation and proinflammatory cytokines estimation).

Autor: Abu-Elnasr Awwad A; Department of otorhinolaryngology, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Hasan RA; Department of Histology and Cell Biology, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Hablas MGA; Department of Histology and Cell Biology, Faculty of Medicine, Suez University, Suez, Egypt., Mohammad Mohammad Abdelhay O; Department of Medical Physiology, Faculty of medicine, Al-Azhar University, Cairo, Egypt., Dawood YMA; Department of otorhinolaryngology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Ahmed Mohamed B; Department of otorhinolaryngology, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Rabou KAA; Department of otorhinolaryngology, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Salem TMM; Department of otorhinolaryngology, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Elhady M; Department of Pediatrics, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Abd El-Aal GN; Department of Pediatrics, Faculty of Medicine for Girls (AFMG), Al-Azhar University, Cairo, Egypt., Ahmed AHE; Department of Pediatrics, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Ibrahim Mostafa Hasan A; Department of Pediatrics, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Elmadbouly AAE; Department of Clinical Pathology, Faculty of Medicine for Girls (Cairo), Al-Azhar University, Cairo, Egypt., Yahia MB; Department of clinical pathology, Faculty of Medicine for Boys (Cairo), Al-Azhar University, Cairo, Egypt., Ashry WMO; Department of Medical Microbiology and Immunology, Damietta Faculty of Medicine (girls), Al-Azhar University, Damietta, Egypt., El Sayed SSMM; Department of Medical Physiology, Faculty of medicine, Al-Azhar University, Cairo, Egypt., Algendy AMM; Department of Medical Physiology, Faculty of medicine, Al-Azhar University, Cairo, Egypt., Alkot AMF; Department of Medical Physiology, Faculty of medicine, Al-Azhar University, Cairo, Egypt., Farag MF; Department of Physiology, Armed Forces College of Medicine, Cairo, Egypt., El Shenawy Emara AAA; Department of Medical Physiology, Faculty of medicine, Al-Azhar University, Cairo, Egypt., Elbayoumy FMAE; Department of Anatomy and Embryology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Ali HF; Department of clinical pathology, Faculty of Medicine for Boys (Cairo), Al-Azhar University, Cairo, Egypt., Aldesoky MM; Department of Medical Microbiology and Immunology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt., Abd-Eltwab RA; Department of Medical Microbiology and Immunology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt., Manawy SM; Department of Anatomy and Embryology, Faculty of Medicine, Benha University, Benha, Egypt., Mohamed Faruk E; Department of Anatomy, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia. faruk_eman@yahoo.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 17; Vol. 13 (1), pp. 8014. Date of Electronic Publication: 2023 May 17.
DOI: 10.1038/s41598-023-33970-x
Abstrakt: Inflammatory processes are increasingly attributed to macrophage polarization. Proinflammatory macrophages promote T helper (Th) 1 response, tissue repair, and Th2 responses. Detection of macrophages in tissue sections is facilitated by CD68. Our study is focused on the expression of CD68 and the estimation of proinflammatory cytokines in children's patients with chronic tonsillitis secondary to vitamin D supplementation. This hospital-based Randomized prospective case-control study was conducted on 80 children with chronic tonsillitis associated with vitamin D deficiency where (40 received vitamin D 50,000 IU weekly for 3-6 months and 40 received 5 ml distilled water as placebo). The serum 25-hydroxyvitamin D [25(OH)D] was measured using an Enzyme-linked immunosorbent assay on all included children. Different histological and immunohistochemical studies for the detection of CD68 were done. There was a significantly lower serum level of 25(OH)D in the placebo group versus the vitamin D group (P < 0.001). The levels of pro-inflammatory cytokines, TNFα, and IL-2 significantly increased in the placebo group as compared to the vitamin D group (P < 0.001). The increased level of IL-4 and IL-10 in the placebo group as compared to the vitamin D group was insignificant (P = 0.32, 0.82) respectively. Vitamin D supplementation alleviated the deleterious effect of chronic tonsillitis on the histological structure of the tonsil. Tonsillar tissues of the children in the control and vitamin D groups demonstrated a highly statistically significantly lower number of CD68 immunoexpressing cells compared with those in the placebo group (P < 0.001). Low vitamin D may play a role in chronic tonsillitis. Vitamin D supplementation could help reduce the occurrence of chronic tonsillitis in susceptible children.
(© 2023. The Author(s).)
Databáze: MEDLINE
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